Hepatitis C virus-related internal ribosome entry sites are found in multiple genera of the family Picornaviridae

被引：45

作者：

Chard, LS

Bordeleau, ME

Pelletier, J

Tanaka, J

Belsham, GJ ^{[1
]}

机构：

[1] BBSRC, Inst Anim Hlth, Woking GU24 0NF, Surrey, England

[2] Dept Biochem, Montreal, PQ H3G 1Y6, Canada

[3] McGill Canc Ctr, Montreal, PQ H3G 1Y6, Canada

[4] Univ Ryukyus, Dept Chem Biol & Marine Sci, Nishihara, Okinawa 9030213, Japan

来源：

JOURNAL OF GENERAL VIROLOGY | 2006年 / 87卷

关键词：

D O I：

10.1099/vir.0.81546-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The internal ribosome entry site (IRES) elements from porcine enterovirus 8 and simian virus 2, two members of a proposed new genus within the family Picornaviridae, were characterized. These IRES elements, in common with the porcine teschovirus 1 IRES, were found to be related functionally and structurally to the IRES element from Hepatitis C virus, a member of the family Flaviviridae. Partial secondary structure predictions were derived and functional assays demonstrated that these IRES elements continued to be active when eIF4G was cleaved and when the activity of eIF4A was blocked.

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页码：927 / 936

页数：10

相关论文

共 31 条

[1] Activity of the hepatitis A virus IRES requires association between the cap-binding translation initiation factor (eIF4E) and eIF4G [J].

Ali, IK ;

McKendrick, L ;

Morley, SJ ;

Jackson, RJ .

JOURNAL OF VIROLOGY, 2001, 75 (17) :7854-7863

[2] Truncated initiation factor eIF4G lacking an eIF4E binding site can support capped mRNA translation [J].

Ali, IK ;

McKendrick, L ;

Morley, SJ ;

Jackson, RJ .

EMBO JOURNAL, 2001, 20 (15) :4233-4242

[3]

Belsham GJ, 2000, COLD SPRING HARBOR M, V39, P869

[4]

BORDELEAU ME, 2006, IN PRESS NAT CHEM BI

[5] Intact eukaryotic initiation factor 4G is required for hepatitis A virus internal initiation of translation [J].

Borman, AM ;

Kean, KM .

VIROLOGY, 1997, 237 (01) :129-136

[6] Functional analyses of RNA structures shared between the internal ribosome entry sites of hepatitis C virus and the picornavirus porcine teschovirus 1 Talfan [J].

Chard, LS ;

Kaku, Y ;

Jones, B ;

Nayak, A ;

Belsham, GJ .

JOURNAL OF VIROLOGY, 2006, 80 (03) :1271-1279

[7] The influence of viral coding sequences on pestivirus IRES activity reveals further parallels with translation initiation in prokaryotes [J].

Fletcher, SP ;

Ali, IK ;

Kaminski, A ;

Digard, P ;

Jackson, RJ .

RNA, 2002, 8 (12) :1558-1571

[8] Pestivirus Internal Ribosome Entry Site (IRES) structure and function:: Elements in the 5′ untranslated region important for IRES function [J].

Fletcher, SP ;

Jackson, RJ .

JOURNAL OF VIROLOGY, 2002, 76 (10) :5024-5033

[9] EUKARYOTIC TRANSIENT-EXPRESSION SYSTEM BASED ON RECOMBINANT VACCINIA VIRUS THAT SYNTHESIZES BACTERIOPHAGE-T7 RNA-POLYMERASE [J].

FUERST, TR ;

NILES, EG ;

STUDIER, FW ;

MOSS, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (21) :8122-8126

[10] RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS [J].

GORMAN, CM ;

MOFFAT, LF ;

HOWARD, BH .

MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (09) :1044-1051

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