Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery

被引：152

作者：

Wroblewska, Liliana ^{[1
]}

Kitada, Tasuku ^{[1
]}

Endo, Kei ^{[2
]}

Siciliano, Velia ^{[1
]}

Stillo, Breanna ^{[1
]}

Saito, Hirohide ^{[2
,3
]}

Weiss, Ron ^{[1
]}

机构：

[1] MIT, Synthet Biol Ctr, Dept Biol Engn, Cambridge, MA 02139 USA

[2] Kyoto Univ, Ctr iPS Cell Res & Applicat, Dept Life Sci Frontiers, Kyoto, Japan

[3] Kyoto Univ, Hakubi Ctr Adv Res, Kyoto, Japan

来源：

NATURE BIOTECHNOLOGY | 2015年 / 33卷 / 08期

基金：

美国国家卫生研究院; 日本科学技术振兴机构; 美国国家科学基金会;

关键词：

NONCYTOPATHIC REPLICATION; GENE-EXPRESSION; REPLICONS; BIOLOGY; DESIGN; SWITCH; VIRUS; CELLS;

D O I：

10.1038/nbt.3301

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Synthetic regulatory circuits encoded in RNA rather than DNA could provide a means to control cell behavior while avoiding potentially harmful genomic integration in therapeutic applications. We create post-transcriptional circuits using RNA-binding proteins, which can be wired in a plug-and-play fashion to create networks of higher complexity. We show that the circuits function in mammalian cells when encoded in modified mRNA or self-replicating RNA.

引用

页码：839 / +

页数：5

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