RNA-DNA Differences Are Generated in Human Cells within Seconds after RNA Exits Polymerase II

被引：45

作者：

Wang, Isabel X. ^{[1
]}

Core, Leighton J. ^{[2
]}

Kwak, Hojoong ^{[2
,4
]}

Brady, Lauren ^{[3
]}

Bruzel, Alan ^{[1
,4
]}

McDaniel, Lee ^{[5
]}

Richards, Allison L. ^{[6
]}

Wu, Ming ^{[4
]}

Grunseich, Christopher ^{[7
]}

Lis, John T. ^{[2
]}

Cheung, Vivian G. ^{[1
,4
,8
,9
]}

机构：

[1] Univ Michigan, Inst Life Sci, Ann Arbor, MI 48109 USA

[2] Cornell Univ, Dept Mol Biol & Genet, Ithaca, NY 14853 USA

[3] Univ Penn, Cell & Mol Biol Grad Program, Philadelphia, PA 19104 USA

[4] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA

[5] Univ Penn, Dept Genet, Philadelphia, PA 19104 USA

[6] Univ Michigan, Human Genet Grad Program, Ann Arbor, MI 48109 USA

[7] NINDS, Neurogenet Branch, NIH, Bethesda, MD 20892 USA

[8] Univ Michigan, Dept Pediat, Ann Arbor, MI 48109 USA

[9] Univ Michigan, Dept Genet, Ann Arbor, MI 48109 USA

来源：

CELL REPORTS | 2014年 / 6卷 / 05期

基金：

美国国家卫生研究院;

关键词：

R-LOOP FORMATION; MESSENGER-RNA; ADENOSINE DEAMINASES; PARALLEL DNA; TRANSCRIPTION; GENE; SENATAXIN; REVEALS; IDENTIFICATION; INITIATION;

D O I：

10.1016/j.celrep.2014.01.037

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

RNA sequences are expected to be identical to their corresponding DNA sequences. Here, we found all 12 types of RNA-DNA sequence differences (RDDs) in nascent RNA. Our results show that RDDs begin to occur in RNA chains similar to 55 nt from the RNA polymerase II (Pol II) active site. These RDDs occur so soon after transcription that they are incompatible with known deaminase-mediated RNA-editing mechanisms. Moreover, the 55 nt delay in appearance indicates that they do not arise during RNA synthesis by Pol II or as a direct consequence of modified base incorporation. Preliminary data suggest that RDD and R-loop formations may be coupled. These findings identify sequence substitution as an early step in cotranscriptional RNA processing.

引用

页码：906 / 915

页数：10

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