A critical comparison of the hemolytic and fungicidal activities of cationic antimicrobial peptides

被引：125

作者：

Helmerhorst, EJ ^{[1
]}

Reijnders, IM ^{[1
]}

van't Hof, W ^{[1
]}

Veerman, ECI ^{[1
]}

Amerongen, AVN ^{[1
]}

机构：

[1] Free Univ Amsterdam, Acad Ctr Dent Amsterdam, Dept Oral Biochem, NL-1081 BT Amsterdam, Netherlands

来源：

FEBS LETTERS | 1999年 / 449卷 / 2-3期

关键词：

hemolytic activity; fungicidal activity; cationic peptide; antimicrobial;

D O I：

10.1016/S0014-5793(99)00411-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The hemolytic and fungicidal activity of a number of cationic antimicrobial peptides was investigated. Histatins and magainins were inactive against human erythrocytes and Candida albicans cells in phosphate buffered saline, but displayed strong activity against both cell types,when tested in I mM potassium phosphate buffer supplemented with 287 mM glucose. The HC50/IC50 ratio, indicative of the therapeutic index, was about 30 for all peptides tested. PGLa was most hemolytic (HC50 = 0.6 mu M) and had the lowest therapeutic index (HC50/IC50 = 0.5), Susceptibility to hemolysis was shown to increase with storage duration of the erythrocytes and also significant differences were found between blood collected from different individuals. In this report, a sensitive assay is proposed for the testing of the hemolytic activities of cationic peptides, This assay detects subtle differences between peptides and allows the comparison between the hemolytic and fungicidal potency of cationic peptides. (C) 1999 Federation of European Biochemical Societies.

引用

页码：105 / 110

页数：6

共 30 条

[1] ANTIMICROBIAL SPECIFICITY AND HEMOLYTIC-ACTIVITY OF CYCLIZED BASIC AMPHIPHILIC BETA-STRUCTURAL MODEL PEPTIDES AND THEIR INTERACTIONS WITH PHOSPHOLIPID-BILAYERS [J].

ANDO, S ;

NISHIKAWA, H ;

TAKIGUCHI, H ;

LEE, S ;

SUGIHARA, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1147 (01) :42-49

[2]

BOMAN HG, 1994, CIBA FDN S, V186

[3] SYNTHETIC MAGAININ ANALOGS WITH IMPROVED ANTIMICROBIAL ACTIVITY [J].

CHEN, HC ;

BROWN, JH ;

MORELL, JL ;

HUANG, CM .

FEBS LETTERS, 1988, 236 (02) :462-466

[4] Peptide helicity and membrane surface charge modulate the balance of electrostatic and hydrophobic interactions with lipid bilayers and biological membranes [J].

Dathe, M ;

Schumann, M ;

Wieprecht, T ;

Winkler, A ;

Beyermann, M ;

Krause, E ;

Matsuzaki, K ;

Murase, O ;

Bienert, M .

BIOCHEMISTRY, 1996, 35 (38) :12612-12622

[5] Hydrophobicity, hydrophobic moment and angle subtended by charged residues modulate antibacterial and haemolytic activity of amphipathic helical peptides [J].

Dathe, M ;

Wieprecht, T ;

Nikolenko, H ;

Handel, L ;

Maloy, WL ;

MacDonald, DL ;

Beyermann, M ;

Bienert, M .

FEBS LETTERS, 1997, 403 (02) :208-212

[6] 3-DIMENSIONAL STRUCTURE OF MEMBRANE AND SURFACE-PROTEINS [J].

EISENBERG, D .

ANNUAL REVIEW OF BIOCHEMISTRY, 1984, 53 :595-623

[7]

GIBSON BW, 1991, J BIOL CHEM, V266, P23103

[8] Synthetic histatin analogues with broad-spectrum antimicrobial activity [J].

Helmerhorst, EJ ;

VantHof, W ;

Veerman, ECI ;

SimoonsSmit, I ;

Amerongen, AVN .

BIOCHEMICAL JOURNAL, 1997, 326 :39-45

[9] INSECT IMMUNITY - PURIFICATION AND PROPERTIES OF 3 INDUCIBLE BACTERICIDAL PROTEINS FROM HEMOLYMPH OF IMMUNIZED PUPAE OF HYALOPHORA-CECROPIA [J].

HULTMARK, D ;

STEINER, H ;

RASMUSON, T ;

BOMAN, HG .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1980, 106 (01) :7-16

[10]

Iwahori A, 1997, BIOL PHARM BULL, V20, P267

← 1 2 3 →