Icariin promotes bone formation via the BMP-2/Smad4 signal transduction pathway in the hFOB 1.19 human osteoblastic cell line

被引：192

作者：

Liang, Wenna ^{[1
]}

Lin, Munan ^{[2
]}

Li, Xihai ^{[3
]}

Li, Candong ^{[1
]}

Gao, Bizheng ^{[1
]}

Gan, Huijuan ^{[1
]}

Yang, Zhaoyang ^{[1
]}

Lin, Xuejuan ^{[1
]}

Liao, Linghong ^{[1
]}

Yang, Min ^{[1
]}

机构：

[1] Fujian Univ Tradit Chinese Med, Res Base Tradit Chinese Med Syndrome, Fuzhou 350122, Peoples R China

[2] Fuzhou Gen Hosp Nanjing Mil Command, Dept Tradit Chinese Med, Fuzhou 350025, Peoples R China

[3] Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou 350122, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2012年 / 30卷 / 04期

基金：

中国国家自然科学基金;

关键词：

bone formation; osteoblast; icariin; osteoporosis; MARROW STROMAL CELLS; IN-VITRO; POSTMENOPAUSAL OSTEOPOROSIS; OSTEOGENIC DIFFERENTIATION; CBFA1; EXPRESSION; DEFICIENT MICE; INVOLVEMENT; FLAVONOIDS; RANKL;

D O I：

10.3892/ijmm.2012.1079

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

Icariin, the main active compound of the traditional Chinese medicine, Epimedium, is commonly used for the clinical treatment of osteoporosis. However, the precise molecular mechanism of the therapeutic effect of icariin has not been elucidated. The aim of this study was to examine the effect of icariin on cell viability, alkaline phosphatase (ALP) activity, the amount of calcified nodules, and to delineate the molecular mechanism of icariin-enhanced bone formation by investigating the expression of bone morphogenic protein-2 (BMP-2), Smad4, Cbfa1/Runx2, osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL) and the OPG/RANKL ratio in the hFOB 1.19 human osteoblastic cell line. We found that icariin significantly increased the cell viability, the activity of ALP and the amount of calcified nodules in the hFOB 1.19 cells. Furthermore, we observed that icariin upregulated the expression of BMP-2, Smad4, Cbfa1/Runx2, OPG, RANKL and the OPG/RANKL ratio. Our results indicate that icariin can modulate the process of bone formation via the BMP-2/Smad4 signal transduction pathway in hFOB 1.19 cells.

引用

页码：889 / 895

页数：7

共 29 条

[1]

Icariin stimulates MC3T3-E1 cell proliferation and differentiation through up-regulation of bone morphogenetic protein-2 [J].