Establishment and properties of neural stem cell clones:: Plasticity in vitro and in vivo

被引:122
作者
Vescovi, AL
Snyder, EY
机构
[1] Harvard Univ, Childrens Hosp, Sch Med, Dept Neurol,Div Neurosci, Boston, MA 02115 USA
[2] Harvard Univ, Childrens Hosp, Sch Med, Dept Pediat,Div Newborn Med, Boston, MA 02115 USA
[3] Harvard Univ, Childrens Hosp, Sch Med, Dept Neurosurg,Div Neurosci Res, Boston, MA 02115 USA
[4] Natl Neurol Inst C Besta, Neuropharmacol Lab, I-20133 Milan, Italy
关键词
D O I
10.1111/j.1750-3639.1999.tb00542.x
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
The study of the basic physiology of the neural precursors generated during brain development is driven by two inextricably linked goals. First, such knowledge is instrumental to our understanding of how the high degree of cellular complexity of the mature central nervous system (CNS) is generated, and how to dissect the steps of proliferation, fate commitment, and differentiation that lead early pluripotent neural progenitors to give rise to mature CNS cells. Second, it is hoped that the isolation, propagation, and manipulation of brain precursors and, particularly, of multipotent neural stem cells (NSCs), will lead to therapeutic applications in neurological disorders,The debate is still open concerning the most appropriate definition of a stem cell and on how it is best identified, characterized, and manipulated. By adopting an operational definition of NSCs, we review some of the basic findings in this area and elaborate on their potential threapeutic applications, Further, we discuss recent evidence from our two groups that describe, based on that rigorous definition, the isolation and propagation of clones of NSCs from the human fetal brain and illustrate how they have begun to show promise for neural cell replacement and molecular support therapy in models of degenerative CNS diseases,The extensive propagation and engraftment potential of human CNS stem cells may, in the not-too-distant-future, be directed towards genuine clinical therapeutic ends, and may open novel and multifaceted strategies for redressing a variety of heretofore untreatable CNS dysfunctions.
引用
收藏
页码:569 / 598
页数:30
相关论文
共 152 条
[1]  
Alvarez-Buylla A, 1998, J NEUROBIOL, V36, P105, DOI 10.1002/(SICI)1097-4695(199808)36:2<105::AID-NEU1>3.0.CO
[2]  
2-5
[3]   FETAL NEURAL GRAFTS AND REPAIR OF THE INJURED SPINAL-CORD [J].
ANDERSON, DK ;
HOWLAND, DR ;
REIER, PJ .
BRAIN PATHOLOGY, 1995, 5 (04) :451-457
[4]   NEURAL-TARGETED GENE-THERAPY FOR RODENT AND PRIMATE HEMIPARKINSONISM [J].
ANTON, R ;
KORDOWER, JH ;
MAIDMENT, NT ;
MANASTER, JS ;
KANE, DJ ;
RABIZADEH, S ;
SCHUELLER, SB ;
YANG, J ;
RABIZADEH, S ;
EDWARDS, RH ;
MARKHAM, CH ;
BREDESEN, DE .
EXPERIMENTAL NEUROLOGY, 1994, 127 (02) :207-218
[5]  
Auguste K. I., 1996, Society for Neuroscience Abstracts, V22, P484
[6]   IMMORTALIZATION OF MOUSE NEURAL PRECURSOR CELLS BY THE C-MYC ONCOGENE [J].
BARTLETT, PF ;
REID, HH ;
BAILEY, KA ;
BERNARD, O .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (09) :3255-3259
[7]   NEUROBIOLOGY - BETTER CELLS FOR BRAIN REPAIR [J].
BJORKLUND, A .
NATURE, 1993, 362 (6419) :414-415
[8]   THE USE OF TRANSPLANTED GLIAL-CELLS TO RECONSTRUCT GLIAL ENVIRONMENTS IN THE CNS [J].
BLAKEMORE, WF ;
OLBY, NJ ;
FRANKLIN, RJM .
BRAIN PATHOLOGY, 1995, 5 (04) :443-450
[9]   Neuronal progenitors as tools for cell replacement in the nervous system [J].
Brustle, O ;
McKay, RDG .
CURRENT OPINION IN NEUROBIOLOGY, 1996, 6 (05) :688-695
[10]   Chimeric brains generated by intraventricular transplantation of fetal human brain cells into embryonic rats [J].
Brüstle, O ;
Choudhary, K ;
Karram, K ;
Hüttner, A ;
Murray, K ;
Dubois-Dalcq, M ;
McKay, RDG .
NATURE BIOTECHNOLOGY, 1998, 16 (11) :1040-1044