Rat epidermal stem cells promote the angiogenesis of full-thickness wounds

被引：24

作者：

Huang, Shaobin ^{[1
,2
]}

Hu, Zhicheng ^{[1
]}

Wang, Peng ^{[1
]}

Zhang, Yi ^{[3
]}

Cao, Xiaoling ^{[1
]}

Dong, Yunxian ^{[1
]}

Cheng, Pu ^{[1
]}

Xu, Hailin ^{[1
]}

Zhu, Wenkai ^{[4
]}

Tang, Bing ^{[1
]}

Zhu, Jiayuan ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Burn, Guangzhou 510080, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 6, Dept Plast Surg, Guangzhou 510080, Guangdong, Peoples R China

[3] Nantong Univ, Affiliated Hosp, Dept Burn & Plast Surg, Nantong, Peoples R China

[4] Portland State Univ, Dept Chem, Portland, OR 97207 USA

来源：

STEM CELL RESEARCH & THERAPY | 2020年 / 11卷 / 01期

基金：

中国国家自然科学基金;

关键词：

ESCs; Cell differentiation; Angiogenesis; Full-thickness wounds; THERAPIES;

D O I：

10.1186/s13287-020-01844-y

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Background Full-thickness wounds severely affect patients' life quality and become challenging problems for clinicians. Stem cells have great prospects in the treatment of wounds. Our previous study confirmed that autologous basal cell suspension could promote wound healing, and epidermal stem cells (ESCs) were detected in the basal cell suspension. Herein, this study aimed to explore the effect of ESCs on full-thickness wounds. Methods Rat ESCs were isolated and expanded and then were transfected with lentivirus to stably express enhanced green fluorescent protein. The experimental rats were randomly divided into 2 groups: in the ESC group, the rat ESCs were sprayed on the full-thickness wounds of rats; in the control group, phosphate-buffered saline was sprayed the on the wounds. Next, wound healing and neovascularization were evaluated. Colonization, division, and differentiation of ESCs on the wound were analyzed by immunofluorescence. Results The rat ESCs colonized, divided, and proliferated in the wound. Additionally, rat ESCs around blood vessels differentiated into vascular endothelial cells and formed a lumen-like structure. Compared with the control group, the ESC group showed enhanced angiogenesis and accelerated wound healing. Conclusions Our study confirmed that rat ESCs are safe and effective for treating full-thickness wounds. Additionally, under certain conditions, ESCs can differentiate into vascular endothelial cells to promote angiogenesis and wound healing.

引用

页数：9

共 25 条

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