C/EBP-β mediates iNOS induction by hypoxia in rat pulmonary microvascular smooth muscle cells

被引：28

作者：

Teng, XW

Li, DC

Catravas, JD

Johns, RA

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD 21287 USA

[2] Med Coll Georgia, Vasc Biol Ctr, Augsburg, Germany

[3] Med Coll Georgia, Dept Pharmacol & Toxicol, Augsburg, Germany

来源：

CIRCULATION RESEARCH | 2002年 / 90卷 / 02期

关键词：

D O I：

10.1161/hh0202.103647

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Exposure of rats to 10% O-2 for 4 days caused pulmonary hypertension and induced expression of both inducible nitric oxide synthase (iNOS) and CCAAT box enhancer binding protein-beta (C/EBP-beta) in rat lung. Electrophoretic mobility shift assays (EMSAs) showed that exposure to 1% O-2 increased the C/EBP-beta binding in rat pulmonary microvascular smooth muscle cells (rPSMs). To test the hypothesis that C/EBP-beta participates in hypoxia-induced iNOS expression in rPSMs, a C/EBP motif at -910 by of rat iNOS promoter was mutated. rPSMs transfected with the rat iNOS promoter and exposed to 1% O-2 for 24 hours had significantly increased wild-type iNOS promoter activity. The hypoxia-induced promoter activity was abolished by the C/EBP motif mutation. Thus, C/EBP-beta mediates, at least in part, hypoxia-induced iNOS expression in rPSMs.

引用

页码：125 / 127

页数：3

共 18 条

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