Effect of denaturation on the photochemistry of pyrimidine bases in isolated DNA

被引：45

作者：

Douki, T ^{[1
]}

机构：

[1] CEA Grenoble, Lab Lesions Acides Nucl, Serv Chim Inorgan & Biol,UMR E3,CEA UJF, CEA DSM Dept Rech Fondamentale Mat Condensee, F-38054 Grenoble 9, France

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2006年 / 82卷 / 01期

关键词：

DNA damage; UV radiation; pyrimidine dimers; DNA photochemistry; ionic strength; DNA structure;

D O I：

10.1016/j.jphotobiol.2005.08.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The influence of denaturation on DNA photochemistry was studied by quantifying the yield of formation of all possible bipyrimidine photolesions within isolated genomic DNA samples exposed to UVC radiation. Effects of DNA melting was studied either by carrying out irradiation over a wide range of temperature (0-90 degrees C) or by decreasing the ionic strength of the solution at 30 degrees C. A first observation was a much larger decrease in the photoreactivity upon increasing the temperature in single-stranded than in double-stranded DNA. Secondly, formation of trans,syn cyclobutane dimers and, to a lesser extent, modification in the ratio between the yields of cyclobutane dimers and (6-4) photoproducts, were found to be other main features associated with denaturation. These results emphasize the modulating role of structure in the yield and nature of UV-induced DNA damage. (C) 2005 Elsevier B.V. All rights reserved.

引用

页码：45 / 52

页数：8

共 51 条

[1] Nearest neighbor thermodynamic parameters for internal G•A mismatches in DNA [J].