Immobilizing proteins on the surface of yeast cells

被引：134

作者：

Schreuder, MP

Mooren, ATA

Toschka, HY

Verrips, CT

Klis, FM

机构：

[1] UNILEVER RES LABS VLAARDINGEN,3133 AT VLAARDINGEN,NETHERLANDS

[2] LANGNESE IGLO GMBH,D-48734 REKEN,GERMANY

来源：

TRENDS IN BIOTECHNOLOGY | 1996年 / 14卷 / 04期

关键词：

D O I：

10.1016/0167-7799(96)10017-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Yeast has a rigid cell wall comprising an outer layer of glycoproteins and an internal skeletal layer of glucan; heterologous proteins can be targeted to the glycoprotein layer and become covalently linked to the glucan skeleton. Yeast is a eukaryote that has 'generally regarded as safe' (GRAS) status, and is easy to cultivate, so it seems ideally suited for applications including the manufacture of recyclable, immobilized, biocatalysts, whole-cell vaccines, the presentation of peptide or antibody libraries, and the presentation of adhesion or metal-binding proteins.

引用

页码：115 / 120

页数：6

共 48 条

[1] ASSEMBLY OF COMBINATORIAL ANTIBODY LIBRARIES ON PHAGE SURFACES - THE GENE-III SITE [J].