Morphologically and functionally intact dendritic cells can be derived from the peripheral blood of aged individuals

被引:109
作者
Steger, MM [1 ]
Maczek, C [1 ]
GrubeckLoebenstein, B [1 ]
机构
[1] AUSTRIAN ACAD SCI,INST BIOMED AGING RES,A-6020 INNSBRUCK,AUSTRIA
关键词
dendritic cells; T lymphocytes ageing; SENIEUR protocol; antigen presentation; tetanus toxoid;
D O I
10.1046/j.1365-2249.1996.d01-790.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Dendritic cells are antigen-presenting cells (APC), which are crucial for the initiation of an immune response. In spite of the well known decline of immune function in old age, no information is yet available on whether dendritic cells are also affected by the ageing process. It was therefore the aim of this study to compare peripheral blood dendritic cells (DC) from old and young healthy individuals. Using granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4, DC were propagated from peripheral blood mononuclear cells (PBMC). The obtained cell populations had a typical dendritic morphology and expressed HLA class I and class II, CD23, CD32, CD40, CD44 and CD54, but not CD3 and CD19. Larger numbers of DC were obtained from old individuals than from young ones in spite of a similar expression pattern of surface molecules. DC from aged persons also survived better under in vitro culture conditions. When tested for their antigen-presenting capacity, DC from young and old individuals were equally effective in inducing the proliferation of tetanus toxoid-specific T cell clones after antigenic stimulation. Peripheral blood DC from aged individuals may thus still function as powerful APC. They may represent useful tools for immunotherapy in the aged.
引用
收藏
页码:544 / 550
页数:7
相关论文
共 38 条
  • [1] IMMUNOGENIC TARGETING OF RECOMBINANT PEPTIDE VACCINES TO HUMAN ANTIGEN-PRESENTING CELLS BY CHIMERIC ANTI-HLA-DR AND ANTISURFACE IMMUNOGLOBULIN-D ANTIBODY FAB FRAGMENTS IN-VITRO
    BAIER, G
    BAIERBITTERLICH, G
    LOONEY, DJ
    ALTMAN, A
    [J]. JOURNAL OF VIROLOGY, 1995, 69 (04) : 2357 - 2365
  • [2] T-CELL ACTIVATION IN THE ELDERLY - EVIDENCE FOR SPECIFIC DEFICIENCIES IN T-CELL ACCESSORY CELL-INTERACTIONS
    BECKMAN, I
    DIMOPOULOS, K
    XU, XN
    BRADLEY, J
    HENSCHKE, P
    AHERN, M
    [J]. MECHANISMS OF AGEING AND DEVELOPMENT, 1990, 51 (03) : 265 - 276
  • [3] RECENT ADVANCES IN THE STUDY OF DENDRITIC CELLS AND FOLLICULAR DENDRITIC CELLS
    CAUX, C
    LIU, YJ
    BANCHEREAU, J
    [J]. IMMUNOLOGY TODAY, 1995, 16 (01): : 2 - 4
  • [4] THE VASCULOPATHY OF AGING
    COOPER, LT
    COOKE, JP
    DZAU, VJ
    [J]. JOURNALS OF GERONTOLOGY, 1994, 49 (05): : B191 - B196
  • [5] T-CELL ANTIGEN RECEPTOR GENES AND T-CELL RECOGNITION
    DAVIS, MM
    BJORKMAN, PJ
    [J]. NATURE, 1988, 334 (6181) : 395 - 402
  • [6] CHANGES IN THE MACROPHAGE FUNCTION WITH AGING
    DELAFUENTE, M
    [J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 1985, 81 (04): : 935 - 938
  • [7] DREXHAGE HA, 1995, 9 INT C IMM SAN FRAN, P3718
  • [8] INCREASED CYTOKINE PRODUCTION IN MONONUCLEAR-CELLS OF HEALTHY ELDERLY PEOPLE
    FAGIOLO, U
    COSSARIZZA, A
    SCALA, E
    FANALESBELASIO, E
    ORTOLANI, C
    COZZI, E
    MONTI, D
    FRANCESCHI, C
    PAGANELLI, R
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1993, 23 (09) : 2375 - 2378
  • [9] GILCHREST BA, 1982, J INVEST DERMATOL, V79, P85, DOI 10.1111/1523-1747.ep12500031
  • [10] T-LYMPHOCYTES AND AGING
    GLOBERSON, A
    [J]. INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, 1995, 107 (04) : 491 - 497