Multi-color genomic in situ hybridization identifies parental chromosomes in somatic hybrids of Diospyros kaki and D-glandulosa

被引：13

作者：

Choi, YA ^{[1
]}

Tao, R ^{[1
]}

Yonemori, K ^{[1
]}

Sugiura, A ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Lab Pomol, Sakyo Ku, Kyoto 6068502, Japan

来源：

HORTSCIENCE | 2002年 / 37卷 / 01期

关键词：

karyotype; multi-color genomic in situ hybridization (MCGISH); somatic hybrid; repetitive DNA sequences; Diospyros;

D O I：

10.21273/HORTSCI.37.1.184

中图分类号：

S6 [园艺];

学科分类号：

0902 ;

摘要：

Multi-color genomic in situ hybridization (MCGISH) was performed for mitotic cells of the somatic hybrids of Diospyros kaki (2n = 6x = 90) and D. glandulosa (2n = 2x = 30). Total DNA of D. kaki and D. glandulosa were isolated and labeled with biotin-16-UTP and digoxigenin (DIG)-11-UTP, respectively. The labeled DNAs were used as probes to differentiate parental chromosomes. The biotin-labeled probe was detected with avidin-rhodamine, and the DIG-labeled probe was detected with anti-DIG-FITC (fluorescein isothiocyanate). Ninety chromosomes from D. kaki that showed reddish-orange and 30 chromosomes from D. glandulosa that showed greenish-yellow were observed under a fluorescence microscope. Some chromosomes showed cross-hybridization with both probes at their terminal or other chromosome regions. These results indicated that MCGISH could be used to analyze genomes of Diospyros species whose chromosomes are small and numerous.

引用

页码：184 / 186

页数：3

共 17 条

[1] SPONTANEOUS EXTENSIVE CHROMOSOME ELIMINATION IN SOMATIC HYBRIDS BETWEEN SOMATICALLY CONGRUENT SPECIES NICOTIANA-TABACUM-L AND ATROPA-BELLADONNA L [J].

BABIYCHUK, E ;

KUSHNIR, S ;

GLEBA, YY .

THEORETICAL AND APPLIED GENETICS, 1992, 84 (1-2) :87-91

[2] The interspecific genome structure of cultivated banana, Musa spp. revealed by genomic DNA in situ hybridization [J].

D'Hont, A ;

Paget-Goy, A ;

Escoute, J ;

Carreel, F .

THEORETICAL AND APPLIED GENETICS, 2000, 100 (02) :177-183

[3]

Doyle JJ, 1987, PHYTOCHEMISTRY B, V19, P11

[4] RFLP analysis and genomic in situ hybridization (GISH) in somatic hybrids and their progeny between Lycopersicon esculentum and Solanum lycopersicoides [J].

Escalante, A ;

Imanishi, S ;

Hossain, M ;

Ohmido, N ;

Fukui, K .

THEORETICAL AND APPLIED GENETICS, 1998, 96 (6-7) :719-726

[5] Characterization of somatic hybrids between Brassica napus and Eruca sativa using species-specific repetitive sequences and genomic in situ hybridization [J].

Fahleson, J ;

Lagercrantz, U ;

Mouras, A ;

Glimelius, K .

PLANT SCIENCE, 1997, 123 (1-2) :133-142

[6]

Fukui Kiichi, 1996, P1

[7] ALLODIPLOID NATURE OF ALLIUM-WAKEGI ARAKI REVEALED BY GENOMIC IN-SITU HYBRIDIZATION AND LOCALIZATION OF 5S AND 18S RDNAS [J].

HIZUME, M .

JAPANESE JOURNAL OF GENETICS, 1994, 69 (04) :407-415

[8] GENOMIC IN-SITU HYBRIDIZATION (GISH) AND RFLP ANALYSIS FOR THE IDENTIFICATION OF ALIEN CHROMOSOMES IN THE BACKCROSS PROGENY OF POTATO(+)TOMATO FUSION HYBRIDS [J].

JACOBSEN, E ;

DEJONG, JH ;

KAMSTRA, SA ;

VANDENBERG, PMMM ;

RAMANNA, MS .

HEREDITY, 1995, 74 :250-257

[9]

Kitamura S, 1997, BREEDING SCI, V47, P67

[10] SIMULTANEOUS DISCRIMINATION OF THE 3 GENOMES IN HEXAPLOID WHEAT BY MULTICOLOR FLUORESCENCE INSITU HYBRIDIZATION USING TOTAL GENOMIC AND HIGHLY REPEATED DNA PROBES [J].

MUKAI, Y ;

NAKAHARA, Y ;

YAMAMOTO, M .

GENOME, 1993, 36 (03) :489-494

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