Circadian tryptophan hydroxylase levels and serotonin release in the suprachiasmatic nucleus of the rat

被引:56
作者
Barassin, S
Raison, S [1 ]
Saboureau, M
Bienvenu, C
Maître, M
Malan, A
Pévet, P
机构
[1] Univ Strasbourg 1, UMR 7518, Strasbourg, France
[2] Fac Med, Inst Chim Biol, INSERM, U338, Strasbourg, France
关键词
circadian rhythm; immunoautoradiography; microdialysis; raphe nuclei;
D O I
10.1046/j.1460-9568.2002.01928.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Serotonin (5-HT) plays an important role in the regulation of the time-keeping system in rodents. In the present study, we have investigated the interplay between the rhythms of 5-HT synthesis and release in the suprachiasmatic nuclei (SCN) of the rat. The quantitative distribution of tryptophan hydroxylase (TpH) protein was used as an index of 5-HT synthesis, in perikarya and terminals areas. In the raphe medianus, the maximal levels of TpH was reached in the early daytime period, followed by a decrease before the onset of darkness. Conversely, in the axon terminals of the SCN the highest levels of TpH were found before the onset of the dark-period. Furthermore, TpH amount in SCN displays variations depending on the anatomical area of the SCN. Extracellular 5-HT peaked at the beginning of the night, as evidenced by in vivo microdialysis in the SCN. The 5-HT metabolite, 5-HIAA, presented a similar pattern, but the acrophase occurred in the middle of the dark period. These results suggest that TpH is transported from the soma to the nerve terminals in which 5-HT is synthesized during daytime. This would fill the intracellular stores of 5-HT to provide for its nocturnal release.
引用
收藏
页码:833 / 840
页数:8
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