Computer-aided development of a high-performance liquid chromatographic method for the determination of hydroxyanthraquinone derivatives in Chinese herb medicine rhubarb

With computer simulation predicting separation in reversed-phase gradient elution, a method to separate and determine five hydroxyanthraquinone derivatives having a wide range of polarity in extract of Chinese herbal medicine rhubarb has been developed. The software DryLab was used to model the retention behavior of the compounds as a function of gradient conditions, based on data from two scouting gradient runs. Under the optimized conditions, i.e. column, Zorbax RX-C-18, 5 Gem, 15x0.46 cm; solvent A, 36 mM triethylamine phosphate (TEAP), pH=2.5; solvent B, ACN; gradient, 36/36/80/80% B at 0/5.5/20.5/25.5 min; flow-rate, 1.00 ml/min; temperature, ambient, the method was successfully applied to monitor the quality of rhubarb from different sources. The effect of sample preparation procedures on extraction efficiency was also examined. (C) 1999 Elsevier Science B.V. All rights reserved.