Diagnostic application of oxidation-reduction potential assay for measurement of oxidative stress: clinical utility in male factor infertility

被引:86
作者
Agarwal, Ashok [1 ]
Roychoudhury, Shubhadeep [1 ,2 ]
Sharma, Rakesh [1 ]
Gupta, Sajal [1 ]
Majzoub, Ahmad [3 ]
Sabanegh, Edmund [3 ]
机构
[1] Cleveland Clin, Amer Ctr Reprod Med, Cleveland, OH 44195 USA
[2] Assam Univ, Dept Life Sci & Bioinformat, Silchar 788011, India
[3] Cleveland Clin, Dept Urol, Cleveland, OH 44195 USA
关键词
clinical test; male infertility; oxidation-reduction potential; oxidative stress; reference value; semen; TOTAL ANTIOXIDANT CAPACITY; SPERM DNA-DAMAGE; HUMAN SPERMATOZOA; FREE-RADICALS; SEMEN; PLASMA; PARAMETERS; CORRELATE; IMPACT; BLOOD;
D O I
10.1016/j.rbmo.2016.10.008
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
The objectives of this study were to: (i) describe a protocol measuring the oxidation-reduction potential (ORP) by MiOXSYS System as an alternative method of seminal oxidative stress (OS) testing; (ii) establish a reference value for static ORP (sORP) to distinguish between controls and male factor infertility patients; (iii) evaluate intra-observer and inter-observer reliability; and (iv) examine association of sORP with sperm parameters predictive of male factor infertility. Elevated levels of sORP were seen in infertile patients (6.22 +/- 1.10 mV/10(6) sperm/ml) compared with controls (1.59 +/- 0.29 mV/10(6) sperm/ml) (P = 0.004). A sORP cut-off value 1.36 mV/10(6) sperm/ml identified normal semen and abnormal semen quality with a sensitivity 69.6%, specificity 83.1%, positive predictive value 85.3% and negative predictive value 65.9%. The test demonstrated strong intra-observer (CV 8.39%) and inter-observer reliability (correlations > 0.97). Higher sORP levels were associated with poor sperm parameters across the fertility status of subjects. Negative correlations were noted with sperm parameters (concentration, total sperm count, motility and morphology) indicating these male infertility parameters are related to OS. In conclusion, the introduction of ORP as a novel clinical test for assessment of OS will help clinicians to better diagnose and manage male factor infertility patients. (C) 2016 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:48 / 57
页数:10
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