Ontogeny of the striatal neurons expressing the D2 dopamine receptor in humans: an in situ hybridization and receptor-binding study

被引:23
作者
Brana, C
Aubert, I
Charron, G
Pellevoisin, C
Bloch, B
机构
[1] UNIV BORDEAUX 2, LAB HISTOL EMBRYOL, UMR 5541, F-33076 BORDEAUX, FRANCE
[2] FAC PHARM, BIOPHYS LAB, INSERM U316, F-37200 TOURS, FRANCE
来源
MOLECULAR BRAIN RESEARCH | 1997年 / 48卷 / 02期
关键词
striatal development; D2; receptor; human fetal brain; hybridization; in situ; receptor autoradiography;
D O I
10.1016/S0169-328X(97)00114-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
D2 dopamine receptor (D2R) gene expression was analyzed by in situ hybridization and D2R ligand autoradiography in the human striatum during ontogeny. D2R mRNA and([H-3]YM-09151-2)-binding sites were detected in the striatum from week 12 of fetal life. At this time, D2R mRNA and binding sites were predominant in the putamen and occured in a pattern of clusters. D2R-binding sites displayed a similar pattern. The signal in the caudate nucleus was weak from weeks 12 to 16. From week 20 of fetal life, D2R mRNA and D2R-binding sites signals became intense in the ventral striatum. At birth, D2R mRNA became homogeneously distributed while D2R-binding sites kept an heterogeneously distribution. Comparative topological and temporal analysis of the D2R, enkephalin and D1 dopamine receptor (D1R) mRNAs showed a distinct developmental pattern for each mRNA. Before birth, the neurons expressing enkephalin and D1R mRNAs were preferentially distributed in the matrix and in the striosomes, respectively, while the neurons expressing D2R mRNA did not display a preferential localization. At birth, high levels of enkephalin mRNA were restricted to the matrix; DIR mRNA level was homogeneous throughout the striatum. D2R mRNA was heterogeneously distributed in the whole striatum with high signals located both in the striosomes and the matrix. These results demonstrate that functional D2R are expressed as early as week 12 in the striatum with a heterogeneous distribution. Our findings also demonstrate that, in contrast to what was expected from similar studies in rodents, D2R mRNA and enkephalin mRNA do not display identical, overlapping expression patterns in striatal neurons during human ontogeny. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:389 / 400
页数:12
相关论文
共 48 条
[1]  
Aubert I, 1997, J COMP NEUROL, V379, P72, DOI 10.1002/(SICI)1096-9861(19970303)379:1<72::AID-CNE5>3.0.CO
[2]  
2-F
[3]  
BALK JH, 1995, NATURE, V377, P424
[4]   HETEROGENEOUS DISTRIBUTION OF DOPAMINE D1 AND D2 RECEPTORS IN THE HUMAN VENTRAL STRIATUM [J].
BERENDSE, HW ;
RICHFIELD, EK .
NEUROSCIENCE LETTERS, 1993, 150 (01) :75-79
[5]   STRIATAL NEURONS EXPRESS INCREASED LEVEL OF DOPAMINE D2 RECEPTOR MESSENGER-RNA IN RESPONSE TO HALOPERIDOL TREATMENT - A QUANTITATIVE INSITU HYBRIDIZATION STUDY [J].
BERNARD, V ;
LEMOINE, C ;
BLOCH, B .
NEUROSCIENCE, 1991, 45 (01) :117-126
[6]   [H-3]SCH-23390 BINDING TO D1 DOPAMINE-RECEPTORS IN THE BASAL GANGLIA OF THE CAT AND PRIMATE - DELINEATION OF STRIOSOMAL COMPARTMENTS AND PALLIDAL AND NIGRAL SUBDIVISIONS [J].
BESSON, MJ ;
GRAYBIEL, AM ;
NASTUK, MA .
NEUROSCIENCE, 1988, 26 (01) :101-119
[7]   ONTOGENY OF THE STRIATAL NEURONS EXPRESSING NEUROPEPTIDE GENES IN THE HUMAN FETUS AND NEONATE [J].
BRANA, C ;
CHARRON, G ;
AUBERT, I ;
CARLES, D ;
MARTINNEGRIER, ML ;
TROUETTE, K ;
FOURNIER, MC ;
VITAL, C ;
BLOCH, B .
JOURNAL OF COMPARATIVE NEUROLOGY, 1995, 360 (03) :488-505
[8]  
Brana C, 1996, J COMP NEUROL, V370, P23, DOI 10.1002/(SICI)1096-9861(19960617)370:1<23::AID-CNE3>3.0.CO
[9]  
2-N
[10]   EXPRESSION OF MESSENGER-RNAS ENCODING DOPAMINE-RECEPTORS IN STRIATAL REGIONS IS DIFFERENTIALLY REGULATED BY MIDBRAIN AND HIPPOCAMPAL-NEURONS [J].
BRENE, S ;
HERRERAMARSCHITZ, M ;
PERSSON, H ;
LINDEFORS, N .
MOLECULAR BRAIN RESEARCH, 1994, 21 (3-4) :274-282