A novel perfusion system for the endothelialisation of PTFE grafts under defined flow

被引：15

作者：

Dunkern, TR ^{[1
]}

Paulitschke, M ^{[1
]}

Meyer, R ^{[1
]}

Büttemeyer, R ^{[1
]}

Hetzer, R ^{[1
]}

Burmester, G ^{[1
]}

Sittinger, M ^{[1
]}

机构：

[1] Charite, Dept Rheumatol, Berlin, Germany

来源：

EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY | 1999年 / 18卷 / 02期

关键词：

prosthesis; endothelialisation; shear stress;

D O I：

10.1016/S1078-5884(99)90829-8

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Objectives: to develop a perfusion system for culturing human endothelial cells on small-diameter PTFE groups under defined pulsatile shear stress. Methods: to benefit from a stronger adhesion of endothelial cells to the substrate, We developed a perfusion system which enables culture of endothelial cells on PTFE grafts to confluence under a wide range of shear stress. We also developed an in situ staining method for the determination of the endothelialisation stage by upper light microscopy. Results: the application of pulsatile flow with high shear stress (6.6 dyn/cm(2), 5 min) to a graft endothelialised under perfusion did riot lend to a disruption of the confluent cell layer. In contrast, a shear stress of 3 dyn/cm(2) applied for 5 min tons sufficient to wash move than 50% of endothelial cells off the PTFE graft when cultured to confluence under static conditions. Conclusions: this technique induces a stronger cell adherence of endothelial cells to a PTFE graft in comparison with grafts endothelialised under static conditions. Endothelialised vascular grafts can be pre-conditioned to defined shear stress values.

引用

页码：105 / 110

页数：6

共 18 条

[1] Photo-immobilization of dipyridamole (Persantin(R)) at the surface of polyurethane biomaterials: Reduction of in vitro thrombogenicity [J].