Combined use of temperature and solvent strength in reversed-phase gradient elution .3. Selectivity for ionizable samples as a function of sample type and pH

被引：83

作者：

Zhu, PL

Dolan, JW

Snyder, LR

Hill, DW

VanHeukelem, L

Waeghe, TJ

机构：

[1] LC RESOURCES INC,WALNUT CREEK,CA 94596

[2] UNIV CONNECTICUT,MICROCHEM LAB,STORRS,CT 06269

[3] UNIV MARYLAND,HORN POINT ENVIRONM LAB,CTR ENVIRONM & ESTUARINE STUDIES,CAMBRIDGE,MD 21613

[4] DU PONT AGR PROD,EXPT STN,WILMINGTON,DE 19880

[5] LANZHOU UNIV,DEPT CHEM,LANZHOU 730000,GANSU,PEOPLES R CHINA

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 756卷 / 1-2期

关键词：

selectivity; column temperature; gradient steepness; gradient elution; benzoic acids; anilines; chlorophylls; peptides; proteins; pesticides; basic drugs;

D O I：

10.1016/S0021-9673(96)00723-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability of temperature and gradient steepness to change band spacing has been investigated for several ionizable samples that include 8 substituted benzoic acids, 9 substituted anilines, 22 basic drugs, 9 structurally-related herbicide impurities, 7 chlorophylls and 72 peptides and proteins. Mobile phase pH was also varied to determine the effect of sample ionization on temperature and gradient-steepness selectivity.

引用

收藏

页码：51 / 62

页数：12

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