The spawning pheromone cysteine-glutathione disulfide ('nereithione') arouses a multicomponent nuptial behavior and electrophysiological activity in Nereis succinea males

被引:39
作者
Ram, JL
Müller, CT
Beckmann, M
Hardege, JD
机构
[1] Wayne State Univ, Dept Physiol, Gordon H Scott Hall Basic Med Sci, Detroit, MI 48201 USA
[2] Univ Wales, Cardiff CF1 3TL, S Glam, Wales
关键词
cysteine-glutathione disulfide; pheromone; spawning behavior; sperm release;
D O I
10.1096/fasebj.13.8.945
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The pheromone nereithione (cysteine-glutathione disulfide), which is released by swimming females of the polychaete Nereis succinea to activate spawning behavior of N. succinea males, has recently been identified and synthesized. Nereithione activates sperm release at less than 10(-6) M, one to two orders of magnitude less than oxidized glutathione or any other glutathione derivative tested. The glutathione fragment gamma-glu-cys inhibited sperm release. Nereithione aroused three components of the male nuptial behavior: circling, sperm release, and accelerated swimming. Electrophysiological activity elicited by nereithione near the sperm release site consisted of initial large spikes, cyclic bursting activity, and small spikes lasting up to a minute and was dose dependent, rapid, reversible, and repeatable. This preparation is an excellent model system for characterizing the receptors and functions of a marine pheromone.
引用
收藏
页码:945 / 952
页数:8
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