Dual effect of alkylresorcinols, natural amphiphilic compounds, upon liposomal permeability

被引:27
作者
Gubernator, J [1 ]
Stasiuk, M [1 ]
Kozubek, A [1 ]
机构
[1] Univ Wroclaw, Inst Biochem & Mol Biol, Dept Lipid & Liposomes, PL-51148 Wroclaw, Poland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1999年 / 1418卷 / 02期
关键词
liposome; alkylresorcinol; bilayer permeability; liposomal encapsulation;
D O I
10.1016/S0005-2736(99)00026-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of 5-n-alkylresorcinols, natural amphiphilic compounds, upon properties of phospholipid vesicles depends on their localization asymmetry. A significant increase of the bilayer permeability is observed when the title compounds are present only in the external medium. When these amphiphiles are preincorporated into the bilayer during its formation, the resulting liposomes effectively encapsulate water-soluble solutes which still remain in liposomes after 25 h. Additionally, the size of liposomes made of alkylresorcinol-phosphatidylcholine mixtures after eight cycles of freezing and thawing only (180-200 nm) is severalfold smaller than the size of vesicles prepared in a similar way from phospholipids only and the resulting liposomes are more homogeneous. These liposomes modified with alkylresorcinols are also stable during 40 day storage at both 4 degrees C and 20 degrees C, in contrast to control liposomes that already strongly aggregate after 10 days. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:253 / 260
页数:8
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