Transcription of the human U2 snRNA genes continues beyond the 3′ box in vivo

被引:52
作者
Cuello, P [1 ]
Boyd, DC [1 ]
Dye, MJ [1 ]
Proudfoot, NJ [1 ]
Murphy, S [1 ]
机构
[1] Univ Oxford, Sir William Dunn Sch Pathol, Chem Pathol Unit, Oxford OX1 3RE, England
基金
英国惠康基金;
关键词
pol II; RNA processing; snRNA; termination; transcription;
D O I
10.1093/emboj/18.10.2867
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The 3' box of the human class II snRNA genes is required for proper 3' processing of transcripts, but how it functions is unclear. Several lines of evidence suggest that termination of transcription occurs at the 3' box and the terminated transcript is then a substrate for processing. However, using nuclear run-on analysis of endogenous genes, we demonstrate that transcription continues for at least 250 nucleotides beyond the 3' box of the U2 genes. Although in vivo footprinting analysis of both the U1 and U2 genes detects no protein-DNA contacts directly over the 3' box, a series of G residues immediately downstream from the 3' box of the U1 gene are clearly protected from methylation by dimethylsulfate, In conjunction with the 3' box of the U1 gene, this in vivo footprinted region causes termination of transcription of transiently transfected U2 constructs, whereas a 3' box alone does not. Taken together, these results indicate that the 3' box is not an efficient transcriptional terminator but may act as a processing element that is functional in the nascent RNA.
引用
收藏
页码:2867 / 2877
页数:11
相关论文
共 61 条
  • [1] THE HIGHLY CONSERVED U SMALL NUCLEAR-RNA 3'-END FORMATION SIGNAL IS QUITE TOLERANT TO MUTATION
    ACH, RA
    WEINER, AM
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (06) : 2070 - 2079
  • [2] ORIENTATION-DEPENDENT TRANSCRIPTIONAL ACTIVATOR UPSTREAM OF A HUMAN U2 SNRNA GENE
    ARES, M
    MANGIN, M
    WEINER, AM
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (07) : 1560 - 1570
  • [3] Intergenic transcription and transinduction of the human beta-globin locus
    Ashe, HL
    Monks, J
    Wijgerde, M
    Fraser, P
    Proudfoot, NJ
    [J]. GENES & DEVELOPMENT, 1997, 11 (19) : 2494 - 2509
  • [4] MAZ-DEPENDENT TERMINATION BETWEEN CLOSELY SPACED HUMAN-COMPLEMENT GENES
    ASHFIELD, R
    PATEL, AJ
    BOSSONE, SA
    BROWN, H
    CAMPBELL, RD
    MARCU, KB
    PROUDFOOT, NJ
    [J]. EMBO JOURNAL, 1994, 13 (23) : 5656 - 5667
  • [5] A BLOCK TO ELONGATION IS LARGELY RESPONSIBLE FOR DECREASED TRANSCRIPTION OF C-MYC IN DIFFERENTIATED HL60 CELLS
    BENTLEY, DL
    GROUDINE, M
    [J]. NATURE, 1986, 321 (6071) : 702 - 706
  • [6] HUMAN-U-1 SMALL NUCLEAR-RNA GENES - EXTENSIVE CONSERVATION OF FLANKING SEQUENCES SUGGESTS CYCLES OF GENE AMPLIFICATION AND TRANSPOSITION
    BERNSTEIN, LB
    MANSER, T
    WEINER, AM
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (09) : 2159 - 2171
  • [7] BERNUES J, 1993, EMBO J, V12, P3575
  • [8] Coupling termination of transcription to messenger RNA maturation in yeast
    Birse, CE
    Minvielle-Sebastia, L
    Lee, BA
    Keller, W
    Proudfoot, NJ
    [J]. SCIENCE, 1998, 280 (5361) : 298 - 301
  • [9] Alternative 3'-end processing of U5 snRNA by RNase III
    Chanfreau, G
    AbouElela, S
    Ares, M
    Guthrie, C
    [J]. GENES & DEVELOPMENT, 1997, 11 (20) : 2741 - 2751
  • [10] FORMATION OF THE 3' END ON U SNRNAS REQUIRES AT LEAST 3 SEQUENCE ELEMENTS
    CILIBERTO, G
    DATHAN, N
    FRANK, R
    PHILIPSON, L
    MATTAJ, IW
    [J]. EMBO JOURNAL, 1986, 5 (11) : 2931 - 2937