Phage antibodies against an unstable hapten: Oxygen sensitive reduced flavin

被引：13

作者：

Bruggeman, YE

Boogert, A

vanHoek, A

Jones, PT

Winter, G

Schots, A

Hilhorst, R

机构：

[1] AGR UNIV WAGENINGEN,DEPT BIOCHEM,WAGENINGEN,NETHERLANDS

[2] AGR UNIV WAGENINGEN,LAB MONOCLONAL ANTIBODIES,WAGENINGEN,NETHERLANDS

[3] AGR UNIV WAGENINGEN,DEPT MOLEC PHYS,WAGENINGEN,NETHERLANDS

[4] MRC,CTR PROT ENGN,CAMBRIDGE,ENGLAND

来源：

FEBS LETTERS | 1996年 / 388卷 / 2-3期

关键词：

phage display; single-chain Fv; reduced flavin; time-resolved fluorescence;

D O I：

10.1016/0014-5793(96)00544-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It is difficult to raise antibodies against haptens and antigens that are unstable under the physiological conditions of the serum, Here we have used a phage antibody library to isolate antibody fragments against oxygen sensitive reduced flavin, by selection of the phage under anaerobic and reducing conditions at pH 5 and a pre-elution step with the oxidized flavin. The binding of the reduced hapten to one of the antibody fragments was characterised by time-resolved polarised fluorescence, and shown to be highly specific for the reduced flavin.

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页码：242 / 244

页数：3

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