Procedures to enhance heat resistance of Rhizobium

被引:9
作者
AbdelGadir, AH
Alexander, M
机构
[1] Laboratory of Soil Microbiology, Dept. Soil, Crop and Atmosph. Sci., Cornell University, Ithaca
基金
美国安德鲁·梅隆基金会;
关键词
alginate; clay; heat resistance; Rhizobium;
D O I
10.1023/A:1004200329070
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Means were sought to enhance the heat resistance of a strain of Rhizobium leguminosarum biovar phaseoli that died rapidly in soil at 43 degrees C. Bacteria were more resistant to high temperatures in soil than broth or phosphate buffer. Starving the cells in phosphate buffer enhanced their heat resistance. Heat-resistant variants were obtained that grew at 45 degrees C. Resistance of the parent culture to inactivation at 43 degrees C was enhanced by immobilizing the cells in Ca alginate, amending the soil with clay or immobilization of the cells in clays. The bacteria survived better at 40 degrees C in soil receiving montmorillonite-immobilized cells than in montmorillonite-amended soil. These results suggest that the tolerance of rhizobia to high temperatures in soils may be enhanced.
引用
收藏
页码:93 / 100
页数:8
相关论文
共 23 条
[1]   THE THERMOPHILIC AEROBIC SPOREFORMING BACTERIA [J].
ALLEN, MB .
BACTERIOLOGICAL REVIEWS, 1953, 17 (02) :125-173
[3]   INFLUENCE OF CLAY-MINERALS, HUMIC ACID AND BACTERIAL CAPSULAR POLYSACCHARIDE ON SURVIVAL OF KLEBSIELLA-AEROGENES EXPOSED TO DRYING AND HEATING IN SOILS [J].
BITTON, G ;
HENIS, Y ;
LAHAV, N .
PLANT AND SOIL, 1976, 45 (01) :65-74
[4]  
BOWEN G. D., 1959, Queensland Agricultural Journal, V16, P177
[5]  
CHATEL D L, 1973, Soil Biology and Biochemistry, V5, P415, DOI 10.1016/0038-0717(73)90068-0
[6]   EFFECT OF HIGH SOIL TEMPERATURES ON NODULATION OF COWPEA, VIGNA-UNGUICULATA [J].
DAY, JM ;
ROUGHLEY, RJ ;
EAGLESHAM, ARJ ;
DYE, M ;
WHITE, SP .
ANNALS OF APPLIED BIOLOGY, 1978, 88 (03) :476-481
[7]  
DROFFNER ML, 1985, J GEN MICROBIOL, V131, P2789
[8]   PROLONGED ENVIRONMENTAL-STRESS VIA A 2 STEP PROCESS SELECTS MUTANTS OF ESCHERICHIA, SALMONELLA AND PSEUDOMONAS THAT GROW AT 54-DEGREES-C [J].
DROFFNER, ML ;
YAMAMOTO, N .
ARCHIVES OF MICROBIOLOGY, 1991, 156 (04) :307-311
[9]   INTERACTION OF PHASEOLUS-VULGARIS WITH THERMOTOLERANT ISOLATES OF RHIZOBIUM-LEGUMINOSARUM BIOVAR PHASEOLI FROM KENYAN SOILS [J].
GITONGA, NM ;
WIDDOWSON, D ;
KEYA, SO .
MIRCEN-JOURNAL OF APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1989, 5 (04) :493-504
[10]   FACTORS AFFECTING HEAT RESISTANCE OF NONSPORING ORGANISMS [J].
HANSEN, NH ;
RIEMANN, H .
JOURNAL OF APPLIED BACTERIOLOGY, 1963, 26 (03) :314-&