Separation of hydroxylated polycyclic aromatic hydrocarbons by micellar electrokinetic capillary chromatography

被引：8

作者：

Krismann, U ^{[1
]}

Kleibohmer, W ^{[1
]}

机构：

[1] INST CHEMO & BIOSENSOR EV,D-48149 MUNSTER,GERMANY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 774卷 / 1-2期

关键词：

buffer composition; cyclodextrin modified micellar electrokinetic capillary chromatography; polynuclear aromatic hydrocarbons;

D O I：

10.1016/S0021-9673(97)00268-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A capillary electrophoretic method for effective separation of hydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) is presented. The effect of urea as secondary modifier on the retention behaviour of 10 hydroxylated PAHs using cyclodextrin modified micellar electrokinetic capillary chromatography (CD-MECC) is investigated. The changes of analyte migration times have been attributed to a ternary analyte-cyclodextrin-urea complex. The separation of 10 common hydroxylated PAHs using the developed CD-MECC method is compared to the results gained by high-performance liquid chromatography (HPLC) separation using common reversed-phase conditions.

引用

页码：193 / 201

页数：9

共 21 条

[1] IMPROVED PROCEDURE FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF MONOHYDROXYLATED PAH METABOLITES IN URINE [J].