Expression profiling and functional characterization of a DREB2-type gene from Populus euphratica

被引：104

作者：

Chen, Jinhuan ^{[2
]}

Xia, Xinli ^{[1
]}

Yin, Weilun ^{[2
]}

机构：

[1] Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China

[2] Beijing Forestry Univ, Coll Forestry, Beijing 100083, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 378卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Populus euphratica; DREB transcription factor; Abiotic stress; High-salt stress; Transgenic tobacco; CIS-ACTING ELEMENT; LOW-TEMPERATURE; TRANSCRIPTION FACTOR; HIGH-SALT; ARABIDOPSIS; DROUGHT; COLD; STRESS; DOMAIN; TOLERANCE;

D O I：

10.1016/j.bbrc.2008.11.071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel DREB (dehydration responsive element binding) gene, designated PeDREB2, was isolated from the desert-grown tree, Populus euphratica. Based on multiple sequence alignment and phylogenetic characterization, PeDREB2 was classified as an A-2 group member of the DREB family. Expression of PeDREB2 was induced by cold, drought, and high salinity, but not by abscisic acid (ABA) treatment. PeDREB2 could bind specifically to DRE elements and was targeted to the nucleus when transiently expressed in onion epidermis cells. 35S promoter-driven expression of PeDREB2 improved salt tolerance in transgenic tobacco and did not cause growth retardation. The results indicate that PeDREB2 functions as a novel transcription factor involved in the response of salt stress and might be useful in improving salt tolerance in transgenic plants. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：483 / 487

页数：5

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