Urinary benzene determination by SPME/GC-MS - A study of variables by fractional factorial design and response surface methodology

被引:23
作者
Prado, C
Garrido, J
Periago, JF
机构
[1] Inst Seguridad & Salud Laboral, E-30120 Murcia, Spain
[2] Univ Publ Navarra, Dept Quim Aplicada, Pamplona 31006, Spain
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 804卷 / 02期
关键词
fractional factorial design; response surface methodology; benzene;
D O I
10.1016/j.jchromb.2004.01.020
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The urinary excretion of the unmetabolized benzene seems to be a very good index for biomonitoring benzene in occupationally exposed people. The use of solid phase microextraction (SPME) offers important advantages for its determination. Several variables can influence the benzene extraction process. Experimental design methodology was used to estimate the influence of the different variables and to evaluate the simultaneous effect of the more significant variables on the benzene extraction. The results showed that sample temperature, sample volume and their interaction were the more significant factors. A model was found that relates the amount of benzene extracted with the studied variables. The more adequate working conditions were: extraction temperature 15 degreesC, incubation time 1 min, extraction time 1 min and 2.5 ml of sample volume. The results indicate that this method is capable of providing sensitive and accurate results for the biomonitoring of benzene in urine. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:255 / 261
页数:7
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