Comprehensive study of several general and type-specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinomas

被引：216

作者：

Baay, MFD

Quint, WGV

Koudstaal, J

Hollema, H

Duk, JM

Burger, MPM

Stolz, E

Herbrink, P

机构：

[1] DIAGNOST CTR SSDZ DELFT,DEPT IMMUNOL & INFECT DIS,2600 GA DELFT,NETHERLANDS

[2] ERASMUS UNIV ROTTERDAM,DEPT DERMATOVENEREOL,ROTTERDAM,NETHERLANDS

[3] DIAGNOST CTR SSDZ,DEPT MOLEC BIOL,DELFT,NETHERLANDS

[4] UNIV GRONINGEN HOSP,DEPT PATHOL,GRONINGEN,NETHERLANDS

[5] UNIV GRONINGEN HOSP,DEPT OBSTET & GYNAECOL,GRONINGEN,NETHERLANDS

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 03期

关键词：

D O I：

10.1128/JCM.34.3.745-747.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have compared the efficacies of three general primer pairs for the detection of human papillomavirus (HPV) DNA in formaldehyde-fixed paraffin-embedded carcinomas. The use of these primer pairs leads to underestimates of the HPV prevalence (BP5/6, 61.1%; CPI/IIG, 57.4%; MY09/11, 46.9%; combined, 72.8%). The efficacy of each primer pair seemed to be inversely correlated to the length of the amplimer produced. By using newly developed type-specific primer pairs (amplimer length, approximately 100 bp), an increase in HPV DNA detection (87.6%) was found.

引用

页码：745 / 747

页数：3

共 20 条

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[2]

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