Fluorescence in situ hybridization for detecting ErbB-2 amplification in breast tumor fine needle aspiration biopsies

被引：45

作者：

Sauter, G

Feichter, G

Torhorst, J

Moch, H

Novotna, H

Wagner, U

Durmuller, U

Waldman, FM

机构：

[1] UNIV SAN FRANCISCO, DEPT LAB MED, DIV MOLEC CYTOMET, SAN FRANCISCO, CA 94117 USA

[2] UNIV BASEL, INST PATHOL, DIV GYNECOL CYTOL, BASEL, SWITZERLAND

[3] UNIV BASEL, INST PATHOL, LAB MOLEC CYTOGENET, BASEL, SWITZERLAND

来源：

ACTA CYTOLOGICA | 1996年 / 40卷 / 02期

关键词：

erbB-2; genes; breast neoplasms; in situ hybridization; fluorescence; aspiration biopsy;

D O I：

10.1159/000333683

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

OBJECTIVE: To evaluate the feasibility of erbB-2 amplification? analysis of fine needle aspiration (FNA) biopsies. STUDY DESIGN: FNA smears and dissociated nuclei from 58 breast cancer samples were examined by dual-labeling fluorescence in situ hybridization (FISH) with probes for centromere 17 and the erbB-2 gene. The results were compared with the outcome of erbB-2 immunohistochemistry. RESULTS: Tumors were categorized according to the erbB-2/centromere 17 signal ratio. There were 23 tumors with high-level amplification, four cases with a low-level erbB-2 gain and 27 tumors with normal erbB-2 content. Four tumors showed an erbB-2 deletion, all in patients less than or equal to 42 years of age. ErbB-2 amplification was strongly associated with positive erbB-2 immunostaining (P < .0001). Comparison of FISH analysis on dissociated cells and on FNA biopsies showed high correspondence (P < .0001). CONCLUSION: FISH allows reliable detection of erbB-2 gene amplification on FNA biopsies.

引用

页码：164 / 173

页数：10

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