Determination of 17α-methyltestosterone in muscle tissues of tilapia, rainbow trout, and salmon using liquid chromatography-Tandem mass spectrometry

被引：16

作者：

Chu, PS ^{[1
]}

Lopez, M ^{[1
]}

Serfling, S ^{[1
]}

Gieseker, C ^{[1
]}

Reimschuessel, R ^{[1
]}

机构：

[1] US FDA, Ctr Vet Med, Laurel, MD 20708 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2006年 / 54卷 / 09期

关键词：

methyltestosterone; tilapia; salmon; trout; LC-MS/MS; method;

D O I：

10.1021/jf052701r

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

An analytical method was developed to quantitate and confirm the presence of 17 alpha- methyltestosterone in the muscles of tilapia, rainbow trout, and salmon. The method employed two liquid- liquid partitioning steps and two solid- phase extraction columns for sample cleanup. The final extracts were analyzed on an isocratic reverse-phase liquid chromatography- tandem mass spectrometry system with atmospheric-pressure chemical ionization in the positive ion mode. The method was validated at levels from 0.40 to 1.6 ng/ g, with MT-d(3) used as an internal standard. The accuracy was between 100% and 110%, and coefficients of variation of < 10% were obtained for all three fish species. Muscle tissues from dosed fish were also assayed to demonstrate the effectiveness of the method for recovering the parent drug.

引用

收藏

页码：3193 / 3198

页数：6

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