Genome-wide reprogramming of regulatory networks, transport, cell wall and membrane biogenesis during arbuscular mycorrhizal symbiosis in Lotus japonicus

被引:233
作者
Guether, Mike [1 ,2 ]
Balestrini, Raffaella [1 ,2 ]
Hannah, Matthew [3 ]
He, Ji [4 ]
Udvardi, Michael K. [4 ]
Bonfante, Paola [1 ,2 ]
机构
[1] Univ Turin, Dept Plant Biol, I-10125 Turin, Italy
[2] CNR, IPP, I-10125 Turin, Italy
[3] Max Planck Inst Mol Plant Physiol, D-14476 Postdam Golm, Germany
[4] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA
关键词
cell wall; Gigaspora margarita; laser microdissection; Lotus japonicus; mycorrhizal functioning; nutrient transporters; perifungal membrane; MEDICAGO-TRUNCATULA; ROOT-SYSTEM; TRANSCRIPTION FACTORS; DISEASE RESISTANCE; L[!text type='JS']JS[!/text]YM4 GENE; PLANT; FUNGI; PHOSPHATE; EXPRESSION; REVEALS;
D O I
10.1111/j.1469-8137.2008.02725.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Arbuscular mycorrhizas (AMs) contribute significantly to soil nutrient uptake in plants. As a consequence of the fungal colonization and of the deep reorganization shown by arbusculated cells, important impacts on root transcriptome are expected. An Affymetrix GeneChip with 50 000 probe-sets and real-time RT-PCR allowed us to detect transcriptional changes triggered in Lotus japonicus by the AM fungus Gigaspora margarita, when arbuscules are at their maximum (28 d postinoculation (dpi)). An early time (4 dpi) was selected to differentiate genes potentially involved in signaling and/or in colonization of outer tissues. A large number (75 out of 558) of mycorrhiza-induced genes code for proteins involved in protein turnover, membrane dynamics and cell wall synthesis, while many others are involved in transport (47) or transcription (24). Induction of a subset (24 genes) of these was tested and confirmed by qRT-PCR, and transcript location in arbusculated cells was demonstrated for seven genes using laser-dissected cells. When compared with previously published papers, the transcript profiles indicate the presence of a core set of responsive genes (25) that seem to be conserved irrespective of the symbiotic partner identity. New Phytologist (2009) 182: 200-212doi: 10.1111/j.1469-8137.2008.02725.x.
引用
收藏
页码:200 / 212
页数:13
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