Characterization of biochemical properties and biological activities of biosurfactants produced by Pseudomonas aeruginosa mucoid and non-mucoid strains isolated from hydrocarbon-contaminated soil samples

被引：87

作者：

Das, K ^{[1
]}

Mukherjee, AK ^{[1
]}

机构：

[1] Tezpur Univ, Dept Mol Biol & Biotechnol, ONGC Ctr Petr Biotechnol, Tezpur 784028, India

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2005年 / 69卷 / 02期

关键词：

D O I：

10.1007/s00253-005-1975-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Biochemical and pharmacological properties of biosuffactants produced at 45 degrees C temperature by Pseudomonas aeruginosa mucoid (M) and non-mucoid (NM) strains, isolated from hydrocarbon-contaminated soil samples, were characterized. Both the strains secreted appreciable amount of biosurfactants (5.0-6.5 g/l), responsible for the reduction of surface tension of the medium from 68 to 29 +/- 0.5 mN/m post 96 h of growth. Maximum yield of biosurfactants was observed following the supplementation of NH4Cl and glycerol as nitrogenous source and carbon source, respectively. These thermostable biosurfactants exhibited strong emulsifying property and could release appreciable amount of oil from saturated sand-pack column. Pharmacological characterization of these biosurfactants revealed that they induced dose-dependent hemolysis and coagulation of platelet-poor plasma but were non-detrimental to chicken lung, liver, heart and kidney tissues. Our study has documented that biosurfactants from P. aeruginosa M and NM strains could be exploited for use in petroleum sectors as well in pharmaceutical industries.

引用

页码：192 / 199

页数：8

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