Novel protein targets for organophosphorus compounds

被引：41

作者：

Richards, P

Johnson, M

Ray, D

Walker, C

机构：

[1] Univ Leicester, MRC, Toxicol Unit, Leicester LE1 9HN, Leics, England

[2] Univ Reading, Dept Anim & Microbial Sci, Reading RG6 6AJ, Berks, England

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1999年 / 119卷

关键词：

acetylcholonesterase; di-isopropyl-phophorofluoridate; DFP; dichlorvos; diazinon; organophosphorus pesticide; paraoxon;

D O I：

10.1016/S0009-2797(99)00064-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inhibition of tritiated di-isopropyl phosphorofluoridate labelling by a range of organophopshorus compounds was used to screen for novel OF-reactive targets in rat-brain homogenates. Analysis of target proteins was conducted by SDS/PAGE and detection of tritiated proteins using a thin layer chromatography (TLC) linear analyser. Two major sites of H-3-DFP labelling were found with relative molecular masses of 30 and 85 kDa. Rates of reaction of these labelling sites with a range of OP compounds were compared to that of acetylcholinesterase. The 30 kDa band was found to be more sensitive to paraoxon, dichlorvos and diazoxon than acetylcholinesterase. The 85 kDa band was found to be more sensitive to dichlorvos and diazoxon than acetylcholinesterase. Neither labelling band reacted with chlorfenvinphos or demeton-s-methyl at significant rates. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：503 / 511

页数：9

共 11 条

[1]

ALDRIDGE WN, 1981, SCI PROG, V67, P131

[2] A NEW AND RAPID COLORIMETRIC DETERMINATION OF ACETYLCHOLINESTERASE ACTIVITY [J].

ELLMAN, GL ;

COURTNEY, KD ;

ANDRES, V ;

FEATHERSTONE, RM .

BIOCHEMICAL PHARMACOLOGY, 1961, 7 (02) :88-&

[3]

GALLO MA, 1991, HDB PESTICIDE TOXICO, P920

[4] A PHOSPHORYLATION SITE IN BRAIN AND DELAYED NEUROTOXIC EFFECT OF SOME ORGANOPHOSPHORUS COMPOUNDS [J].

JOHNSON, MK .

BIOCHEMICAL JOURNAL, 1969, 111 (04) :487-&

[5]

KHYSEANDERSON J, 1984, J BIOCHEM BIOPH METH, V10, P203

[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[7]

O'Neill J J, 1981, Fundam Appl Toxicol, V1, P154, DOI 10.1016/S0272-0590(81)80052-8

[8]

OBRIEN SJ, 1995, LANCET, V345, P1631

[9] THE EFFECT OF ORGANOPHOSPHORUS COMPOUNDS ON SERUM PSEUDOCHOLINESTERASE LEVELS IN A GROUP OF INDUSTRIAL-WORKERS [J].

PEEDICAYIL, J ;

ERNEST, K ;

THOMAS, M ;

KANAGASABAPATHY, AS ;

STEPHEN, PM .

HUMAN & EXPERIMENTAL TOXICOLOGY, 1991, 10 (04) :275-278

[10] NEUROPSYCHOLOGICAL EFFECTS OF LONG-TERM EXPOSURE TO ORGANOPHOSPHATES IN SHEEP DIP [J].

STEPHENS, R ;

SPURGEON, A ;

CALVERT, IA ;

BEACH, J ;

LEVY, LS ;

BERRY, H ;

HARRINGTON, JM .

LANCET, 1995, 345 (8958) :1135-1139

← 1 2 →