Somatostatin receptor subtype 2A expression in the rat retina

被引:42
作者
Johnson, J
Wu, V
Wong, H
Walsh, JH
Brecha, NC
机构
[1] Univ Calif Los Angeles, Sch Med, Dept Neurobiol, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, Sch Med, CURE, Div Digest Dis, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, Sch Med, Jules Stein Eye Inst, Los Angeles, CA 90095 USA
[4] Vet Adm Med Ctr W Los Angeles, Los Angeles, CA 90073 USA
关键词
photoreceptors; bipolar cells; amacrine cells; horizontal cells; neuropeptide receptors; visual system;
D O I
10.1016/S0306-4522(99)00170-0
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Somatostatin is mainly expressed by sparsely occurring amacrine and interplexiform cells in the retina. In this study, we characterized the expression and cellular localization of one of the somatostatin subtype (sst) receptors, sst(2A), in the rat retina. The presence of sst(2A) receptor messenger RNA in retinal extracts was demonstrated by reverse transcription-polymerase chain reaction using specific primers to detect the sst(2) receptor and its isoforms, sst(2A) and sst(2B-) Specific sst(2A) receptor immunoreactivity was mainly localized to the plasma membrane of several neuronal cell types. In the outer retina, immunoreactivity was localized to cone photoreceptors, horizontal cells, and rod and cone bipolar cells. Double-label experiments showed the co-localization of sst(2A) receptor and protein kinase C (alpha and beta), a rod bipolar cell marker, and of sst(2A) receptor and Calbindin-D-28k, a horizontal cell marker. In the inner retina, sst(2A) receptor immunoreactivity occurred in tyrosine hydroxylase-positive amacrine cells; most were of medium to large size. These findings indicate that somatostatin may act at a distance, in a paracrine manner, on several cell types that express the sst(2A) receptor, and therefore exert a broad modulatory influence on both scotopic and photopic visual pathways. (C) 1999 IBRO. Published by Elsevier Science Ltd.
引用
收藏
页码:675 / 683
页数:9
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