Cloning, characterization, and localization of mouse and human SPO11

被引：125

作者：

Romanienko, PJ ^{[1
]}

Camerini-Otero, RD ^{[1
]}

机构：

[1] NIDDK, Genet & Biochem Branch, NIH, Bethesda, MD 20892 USA

来源：

GENOMICS | 1999年 / 61卷 / 02期

关键词：

D O I：

10.1006/geno.1999.5955

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Spo11 is a meiosis-specific protein in yeast that has been found covalently bound to DNA double-strand breaks (DSBs) during the early stages of meiosis. These DSBs initiate homologous recombination, which is required for proper segregation of chromosomes and the generation of genetic diversity during meiosis. Here we report the cloning, characterization, tissue expression, and chromosomal localization of both mouse and human homologues of Spo11. The putative mouse and human proteins are 82% identical and share approximately 25% identity with other family members. Northern blot analysis revealed testis-specific expression for both genes, but RT-PCR results showed ubiquitous expression of at least a portion of Spo11 in mouse. Human SPO11 was also detected in several somatic tissues. Mouse Spo11 was localized to chromosome 2H4, and human SPO11 was localized to chromosome 20q13.2-q13.3, a region amplified in some breast and ovarian tumors.

引用

页码：156 / 169

页数：14

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