Vitamin C facilitates pluripotent stem cell maintenance by promoting pluripotency gene transcription

被引：39

作者：

Gao, Yuan ^{[1
,2
]}

Yang, Liping ^{[2
]}

Chen, Linlin ^{[1
,2
]}

Wang, Xiangxue ^{[2
]}

Wu, Haibo ^{[1
]}

Ai, Zhiying ^{[2
]}

Du, Juan ^{[2
]}

Liu, Ye ^{[2
]}

Shi, Xiaoyan ^{[2
]}

Wu, Yongyan ^{[1
]}

Guo, Zekun ^{[1
]}

Zhang, Yong ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Vet Med, Key Lab Anim Biotechnol, Minist Agr, Yangling 712100, Shaanxi, Peoples R China

[2] Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China

来源：

BIOCHIMIE | 2013年 / 95卷 / 11期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

Vitamin C; iPSCs; Reprogramming; Pluripotency; Nanog; NF-KAPPA-B; EMBRYONIC STEM; EXPRESSION; MOUSE; GENERATION; ACTIVATION; INHIBITOR; OCT4; MYC;

D O I：

10.1016/j.biochi.2013.08.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Vitamin C has recently received attention because of its ability to improve induced pluripotent stem cells (iPSCs) generation [1-3] and maintain a blastocyst-like state in ES cells [4]. However, the underlying mechanisms are not fully understood. In this study, we found that vitamin C maintained the morphology of mouse embryonic stem cell (mESC) colonies and inhibited mESC differentiation. Gene expression profiling revealed that the genes down-regulated by vitamin C were grouped in the regulation of differentiation and development, while most of the up-regulated genes were enriched in the regulation of transcription involving numerous pluripotency factors, which was further confirmed by real time quantitative PCR. For the key pluripotency factor Nanog, vitamin C increased its promoter activity and protein level. In addition, pathway screening indicated that vitamin C may affect various signaling pathways. Our study provides new insights into vitamin C-mediated pluripotency maintenance of mESCs. (C) 2013 Elsevier Masson SAS. All rights reserved.

引用

页码：2107 / 2113

页数：7

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