ER degradation;
nuclear envelope;
yeast 26S proteasome;
D O I:
10.1023/A:1006973803960
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
26S proteasomes are multisubunit protease complexes that play the central role in the ubiquitin-dependent protein degradation pathway. The proteolytically active core is formed by the 20S proteasome. Regulatory subunits, principally the 19S cap complex, confer the specificity towards ubiquitinated substrates and an ATP-dependence on proteolysis. Green fluorescence protein (GFP)-tagged versions of either an alpha-subunit of the 20S core or an ATPase subunit of the 19S cap complex were functionally incorporated into the protease complex, thus allowing to monitor the subcellular distribution of 26S proteasomes in living yeast. Our localization studies suggest that proteasomal proteolysis mainly occurs at the nuclear envelope (NE)/rough ER. Implications of proteasomal functions at the NE/rough ER are discussed in the context of published work on ER degradation and with regard to possible targeting mechanisms.
机构:NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
Dai, RM
Chen, EY
论文数: 0引用数: 0
h-index: 0
机构:NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
Chen, EY
Longo, DL
论文数: 0引用数: 0
h-index: 0
机构:NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
Longo, DL
Gorbea, CM
论文数: 0引用数: 0
h-index: 0
机构:NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
Gorbea, CM
Li, CCH
论文数: 0引用数: 0
h-index: 0
机构:
NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USANCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
机构:
ROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USAROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USA
ENENKEL, C
BLOBEL, G
论文数: 0引用数: 0
h-index: 0
机构:
ROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USAROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USA
BLOBEL, G
REXACH, M
论文数: 0引用数: 0
h-index: 0
机构:
ROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USAROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USA
机构:
Technion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, IsraelTechnion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, Israel
Hershko, A
Ciechanover, A
论文数: 0引用数: 0
h-index: 0
机构:Technion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, Israel
机构:NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
Dai, RM
Chen, EY
论文数: 0引用数: 0
h-index: 0
机构:NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
Chen, EY
Longo, DL
论文数: 0引用数: 0
h-index: 0
机构:NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
Longo, DL
Gorbea, CM
论文数: 0引用数: 0
h-index: 0
机构:NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
Gorbea, CM
Li, CCH
论文数: 0引用数: 0
h-index: 0
机构:
NCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USANCI, Intramural Res Support Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA
机构:
ROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USAROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USA
ENENKEL, C
BLOBEL, G
论文数: 0引用数: 0
h-index: 0
机构:
ROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USAROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USA
BLOBEL, G
REXACH, M
论文数: 0引用数: 0
h-index: 0
机构:
ROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USAROCKEFELLER UNIV, HOWARD HUGHES MED INST, CELL BIOL LAB, NEW YORK, NY 10021 USA
机构:
Technion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, IsraelTechnion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, Israel
Hershko, A
Ciechanover, A
论文数: 0引用数: 0
h-index: 0
机构:Technion Israel Inst Technol, Fac Med, Biochem Unit, IL-31096 Haifa, Israel