Salt-independent adsorption chromatography: new broad-spectrum affinity methods for protein capture

被引：54

作者：

Burton, SC ^{[1
]}

Harding, DRK

机构：

[1] Univ Birmingham, Sch Chem Engn, Ctr Bioproc Engn, Biochem Recovery Grp, Birmingham B15 2TT, W Midlands, England

[2] Massey Univ, Inst Fundamental Sci, Palmerston North, New Zealand

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2001年 / 49卷 / 1-3期

关键词：

salt-independent; expanded-bed adsorption; mixed mode; hydrophobic charge induction; immunoglobulin; pH elution;

D O I：

10.1016/S0165-022X(01)00204-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of chromatography in capture is reviewed in terms of the special requirements imposed by the processing of very crude feedstocks. Adsorption methods which are not significantly affected by variations of feedstock ionic strength are highlighted. Methods are compared in terms of simplicity, robustness, selectivity and ease of elution. The application of such methods to enzyme and antibody purifications is summarised. Particular emphasis is placed on high ligand density methods, which have potential for broad-spectrum application. (C) 2001 Published by Elsevier Science B.V.

引用

页码：275 / 287

页数：13

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