Neuroprotective effects of the Chinese Yi-Qi-Bu-Shen recipe extract on injury of rat hippocampal neurons induced by hypoxia/reoxygenation

被引：28

作者：

Liu, De-shan ^{[1
]}

Zhou, Yong-hong ^{[2
]}

Liang, Er-shun ^{[1
]}

Li, Wei ^{[1
]}

Lin, Wei-wei ^{[1
]}

Chen, Fei-fei ^{[1
]}

Gao, Wei ^{[1
]}

机构：

[1] Shandong Univ, Qilu Hosp, Dept Tradit Chinese Med, Jinan 250012, Peoples R China

[2] Qingdao Univ, Sch Med, Inst Tradit Chinese Med, Qingdao 266021, Peoples R China

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2013年 / 145卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Hippocampal neurons; Hypoxia/reoxygenation; Yi-Qi-Bu-Shen recipe; Anti-oxidation; Apoptosis; PEROXIDE-INDUCED APOPTOSIS; ASTRAGALUS-MEMBRANACEUS; OXIDATIVE STRESS; CEREBRAL-ISCHEMIA; PC12; CELLS; PUERARIN; TETRAMETHYLPYRAZINE; PROTECTS; INHIBITION; MECHANISM;

D O I：

10.1016/j.jep.2012.10.046

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Objective: To explore the protective effects of the Chinese Yi-Qi-Bu-Shen recipe (YB) against neuronal injury induced by hypoxia-reoxygenation, which has shown beneficial effect in improving the brain function of type 2 diabetics likely through its antihyperglycemic, antioxidant activity, and investigate its mechanisms. Methods: The bilateral hippocampus was collected from newborn rats to establish single cell suspension. On the 10th day, the primarily cultured hippocampal neurons were randomly divided into five groups: the normal group (NG), the hypoxia/reoxygenation group (HG), and groups protected with small, medium and large dosages of YB (SG, MG and LG, respectively). The YB-protected groups were treated with different concentrations of YB containing serum before reoxygenation. The metabolic rate of MTT, the malondialdehyde (MDA) content, and the activity of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) were measured with assay kits. The apoptosis rate of hippocampal neurons were tested using flow cytomety analysis. RT-PCR was used to evaluate the mRNA expressions of bcl-2 and bax genes. Results: The SOD activity, the cell survival rate, the bcl-2/bax ratio, and the bcl-2 mRNA expression in the HG group were significantly lower (all P < 0.01), but the levels of MDA and LDH, the apoptosis rate, and the bax mRNA expression were higher (all P < 0.01) than those in the NG group. The SOD activity, the cell survival, the bcl-2 mRNA expression, and the bcl-2/bax ratio were significantly higher in all of the YB-protected groups (all P < 0.01), but the level of MDA and LDH, the apoptosis rate, and the bax mRNA expression were lower (P < 0.01, P < 0.05) than those in the HG group in a dose dependent manner. Conclusion: The YB extract has a protective effect on hippocampal neurons against injury induced by hypoxia/reoxygenation, through its antioxidant activity and the regulation of apoptosis. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：168 / 174

页数：7

共 58 条

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In vivo antioxidative activity of a quantified Pueraria lobata root extract [J].