Cellular localization of IL-18 and IL-18 receptor in pig anterior pituitary gland

被引：14

作者：

Nagai, Y

Watanabe, K

Aso, H

Ohwada, S

Muneta, Y

Yamaguchi, T

机构：

[1] Tohoku Univ, Grad Sch Agr Sci, Lab Funct Morphol, Aoba Ku, Sendai, Miyagi 9818555, Japan

[2] Natl Inst Anim Hlth, Dept Immunol, Tsukuba, Ibaraki 3050856, Japan

来源：

DOMESTIC ANIMAL ENDOCRINOLOGY | 2006年 / 30卷 / 02期

关键词：

interleukin-18; interleukin-18R; pig anterior pituitary gland; immuno-endocrine signalling; paracrine;

D O I：

10.1016/j.domaniend.2005.07.008

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Pro-inflammatory cytokine interleukin 18 (IL-18) has been proposed to have a role in modulating immuno-endocrine functions. Our previous study showed that IL-18 and IL-18 receptor (IL-18R) colocalized in somatotrophs of the bovine anterior pituitary gland, and the possibility that IL-18 acts on somatotrophs as an autocrine factor. In the present study, we investigated the localization of IL-18 and IL-18R in the pig anterior pituitary gland. RT-PCR analysis showed the expression of IL-18 and IL-18R mRNAin the pig anterior pituitary gland. Immunohistochemistry of IL-18 and specific hormones revealed the presence of IL-18 in somatotrophs, mammotrophs, thyrotrophs and gonadotrophs. IL-18R was localized in somatotrophs and thyrotrophs. Furthermore, the somatotrophs immunoreactive for IL-18 did not contain IL-18R. Thus, IL-18R and IL-18 were not colocalized in an identical somatotroph. These findings suggest that the localization of IL-18 in pig somatotrophs is different from that in bovine somatotrophs, although IL-18 closely associates with somatotrophs in the anterior pituitary glands in both species. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：144 / 154

页数：11

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