Inverse correlation of epidermal growth factor receptor messenger RNA induction and suppression of anchorage-independent growth by OSI-774, an epidermal growth factor receptor tyrosine kinase inhibitor, in glioblastoma multiforme cell lines

被引:56
作者
Halatsch, ME
Gehrke, EE
Vougioukas, VI
Bötefür, IC
Borhani, FA
Efferth, T
Gebhart, E
Domhof, S
Schmidt, U
Buchfelder, M
机构
[1] Univ Gottingen, Klin & Poliklin Neurochirurg, D-37075 Gottingen, Germany
[2] Univ Gottingen, Abt Med Stat, D-37075 Gottingen, Germany
[3] Univ Freiburg, Abt Allgemeine Neurochirurg, D-7800 Freiburg, Germany
[4] Univ Freiburg, Klin & Poliklin Neurol, D-7800 Freiburg, Germany
[5] Univ Heidelberg, Zentrum Mol Biol, D-6900 Heidelberg, Germany
[6] Univ Erlangen Nurnberg, Inst Humangenet, D-8520 Erlangen, Germany
关键词
glioblastoma multiforme; epidermal growth factor receptor; tyrosine kinase inhibitor; proliferation; anchorage-independent growth; apoptosis;
D O I
10.3171/jns.2004.100.3.0523
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Object. Quantitative and qualitative alterations in the epidermal growth factor receptor (EGFR) commonly occur in many cancers in humans, including malignant gliomas. The aim of the current study was to evaluate molecular and cellular effects of OSI-774, a novel EGFR tyrosine kinase inhibitor, on nine glioblastoma multiforme (GBM) cell lines. Methods. The effects of OSI-774 on expression of EGFR messenger (m)RNA and protein, proliferation, anchorage-independent growth, and apoptosis were examined using semiquantitative reverse transcription-polymerase chain reaction, immunocytochemical analysis, Coulter counting, soft agar cloning, and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling/fluorescence-activated cell sorting, respectively. All p53 genes were completely and bidirectionally sequenced. Suppression of anchorage-independent growth by OSI-774 was inversely correlated to the induction of EGFR mRNA during relative serum starvation (r = -0.74) and was unrelated to p53 status. Overall, suppression of anchorage-independent growth was a considerably stronger effect of OSI-774 than inhibition of proliferation. The extent of OSI-774-induced apoptosis positively correlated with both proliferation and anchorage-independent growth of GBM cell lines (r = 0.75 and 0.79, respectively). In a single cell line derived from a secondary GBM, exposure to concentrations of greater than or equal to 1 mumol/L resulted in a substantial net cell loss during proliferation studies. Conclusions. The induction of EGFR mRNA may constitute a cellular mechanism to counteract the inhibitory effect of OSI-774 on the anchorage-independent growth of GBM cells. In contrast, no considerable correlation could be established between baseline expression levels of EGFR (both mRNA and protein) in GBM cell lines and their biological response to OSI-774. The OSI-774 induced greater (p53-independent) apoptosis in more malignant GBM phenotypes and may be a promising therapeutic agent against secondary GBM.
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页码:523 / 533
页数:11
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