Laminarin Induces Apoptosis of Human Colon Cancer LOVO Cells through a Mitochondrial Pathway

被引:46
作者
Ji, Yu Bin [2 ]
Ji, Chen Feng [1 ]
Zhang, He [1 ]
机构
[1] Harbin Univ Commerce, Ctr Res Life Sci & Environm Sci, Harbin 150076, Peoples R China
[2] Harbin Univ Commerce, Engn Res Ctr Nat Anticancer Drugs, Minist Educ, Harbin 150076, Peoples R China
来源
MOLECULES | 2012年 / 17卷 / 08期
关键词
laminarin; human colon cancer; apoptosis; mitochondrial pathway; ACTIVATION;
D O I
10.3390/molecules17089947
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Many scientific studies have shown that laminarin has anti-tumor effects, but the anti-tumor mechanism was unclear. The purpose of this study was to investigate the effect of laminarin on the induction of apoptosis in human colon cancer LOVO cells and the molecular mechanism involved. LOVO cells were treated with different concentrations of laminarin at different times. Morphology observations were performed to determine the effects of laminarin on apoptosis of LOVO cells. Flow cytometry (FCM) was used to detect the level of intracellular reactive oxygen species (ROS) and pH. Laser scanning confocal microscope (LSCM) was used to analyze intracellular calcium ion concentration, mitochondrion permeability transition pore (MPTP) and mitochondrial membrane potential (MMP). Western blotd were performed to analyze the expressions of Cyt-C, Caspase-9 and -3. The results showed the apoptosis morphology, which showed cell protuberance, concentrated cytoplasm and apoptotic bodies, was obvious after 72 h treatment. Laminarin treatment for 24 h increased the intracellular level of ROS and Ca2+; decreased pH value; activated intracellular MPTP and decreased MMP in dose-dependent manners. It also induced the release of Cyt-C and the activation of Caspase-9 and -3. In conclusion, laminarin induces LOVO cell apoptosis through a mitochondrial pathway, suggesting that it could be a potent agent for cancer prevention and treatment.
引用
收藏
页码:9947 / 9960
页数:14
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