Expression of brain subtype creatine kinase in the zebrafish embryo

被引:15
作者
Dickmeis, T
Rastegar, S
Aanstad, P
Clark, M
Fischer, N
Plessy, C
Rosa, F
Korzh, V
Strähle, U
机构
[1] ULP, CU Strasbourg, INSERM, CNRS,Inst Genet & Biol Mol & Cellulaire, F-67404 Illkirch Graffenstaden, France
[2] Max Planck Inst Mol Genet, D-14195 Berlin, Dahlem, Germany
[3] Ecole Normale Super, INSERM, U368, F-75230 Paris 05, France
[4] Natl Univ Singapore, Inst Mol Agrobiol, Singapore 117548, Singapore
关键词
gastrulation; neuronal differentiation; maternal transcript; energy shuttle; primary neurons; zebrafish; embryogenesis; limb; somite;
D O I
10.1016/S0925-4773(01)00536-6
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Creatine kinases (CK) play crucial roles in intracellular energy transfer. We have isolated a cDNA from zebrafish embryos, which encodes a CK highly related to the mammalian brain subtype creatine kinase (BCK). The bck mRNA is expressed maternally in the zebrafish embryo and transcripts are distributed uniformly in blastula and gastrula stages. Expression becomes restricted to the prechordal plate and the nervous system during subsequent somitogenesis stages. bck transcripts are abundant in primary neurons in the developing central nervous system of the 1-day-old embryo. While some bck expression persists in the hindbrain, expression vanishes in the spinal cord of the 2-day-old embryo. In summary, the expression pattern of lick is highly dynamic and suggests a role for bck during gastrulation and neuronal differentiation. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:409 / 412
页数:4
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