Cell line-specific transcriptional activation of the promoter of the human guanylyl cyclase C heat-stable enterotoxin receptor gene

被引：16

作者：

Mann, EA

Jump, ML

Giannella, RA

机构：

[1] UNIV CINCINNATI,COLL MED,DIV DIGEST DIS,CINCINNATI,OH 45267

[2] VET AFFAIRS MED CTR,CINCINNATI,OH 45220

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1996年 / 1305卷 / 1-2期

关键词：

guanylyl cyclase C; gene expression; intestine; Caco-2;

D O I：

10.1016/0167-4781(95)00190-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The guanylyl cyclase C protein, expressed primarily in the intestine, is the receptor for the heat-stable enterotoxin of Escherichia coli. We have isolated and sequenced the promoter region and the first exon of human guanylyl cyclase C and determined the major site of transcription initiation. Transfection of a -1973/+124 promoter/luciferase gene fusion construct in the Caco-2 intestinal cell line resulted in a high level of expression; results with deletion constructs indicate the presence of multiple positive-acting sequence elements. These promoter elements were not active upon transfection into MH/3T3 and LLC-PK1 cell lines which do not express GC-C.

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页码：7 / 10

页数：4

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