Detection of the response regulator AgrA in the cytosolic fraction of Staphylococcus aureus by monoclonal antibodies

被引：32

作者：

Morfeldt, E

PanovaSapundjieva, I

Gustafsson, B

Arvidson, S

机构：

[1] Microbiology and Tumorbiology Center, Karolinska Institutet, Box 280

[2] Transport Medical Insitute, Sofia

[3] Pharmacia AB, S-112 87 Stockholm

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 143卷 / 2-3期

关键词：

Staphylococcus aureus; Agr; response regulator; regulation; virulence genes;

D O I：

10.1016/0378-1097(96)00310-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The expression of many virulence genes in Staphylococcus aureus is controlled by a regulatory RNA molecule, RNAIII, which is encoded by the agr locus. Transcription of RNAIII requires the activity of the agrA, B, C and D genes, which code for components of a quorum sensing signal transduction system. In this report we describe the overexpression and purification of the response regulator, AgrA Monoclonal antibodies were produced and used to detect AgrA in the cytosolic fraction of S. aureus cells. Purified AgrA did not bind to the RNAIII promoter region in a DNA mobility shift experiment. This confirms previous results obtained with protein extracts from agr(+) and agr(-) cells.

引用

页码：195 / 201

页数：7

共 25 条

[1] ANALYSIS OF GENE-CONTROL SIGNALS BY DNA-FUSION AND CLONING IN ESCHERICHIA-COLI [J].