Calcyclin binding protein promotes DNA synthesis and differentiation in rat neonatal cardiomyocytes

被引：44

作者：

Au, Ka-Wing

Kou, Cecy Y. C.

Woo, Anthony Y. H.

Chim, Stephen S. C.

Fung, Kwok-Pui

Cheng, Christopher H. K.

Waye, Mary M. Y.

Tsui, Stephen K. W.

机构：

[1] Chinese Univ Hong Kong, Dept Biochem, Shatin, Hong Kong, Peoples R China

[2] Chinese Univ Hong Kong, Croucher Lab Human Genom, Shatin, Hong Kong, Peoples R China

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2006年 / 98卷 / 03期

关键词：

CacyBP/SIP; DNA synthesis; differentiation; H9C2; cardiomyocytes;

D O I：

10.1002/jcb.20710

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

During cardiac muscle devolopment, most cardiomyocytes permanently withdraw from the cell cycle. Previously, by suppressive subtractive hybridization, we identified calcylclin-binding protein/Siah-interacting protein important role of CacyBP/SIP in cardiac devolopment. To show the importance of CacyBP/SIP during myoblast differentiation, we report here that cacyBP/SIP is devolopmentally regulated in postnatal rat hearts. The overexpression of CacyBp/SIP promotes the differentiation and DNA synthesis of H9C2 cells and primary rat cardiomyocytes, as well as downregulates the expression of beta-catenin. Besides, CacyBP/SIP promotes the formation of myotubes and multinucleation upon differentiation. To investigate the cardioprotective role of CacyBP/SIP in cardiomyocytes, a hypoxia/reoxygenation model was employed. We found that CacyBP/SIP was upregulated during myocardial infarction (MI) and hypoxia/reoxygenation. As a conclusion, CacyBP/SIP may play a role in cardiomyogenic differentiation and possibly protection of cardiomyocytes during hypoxia/reoxygenation injury.

引用

页码：555 / 566

页数：12

共 24 条

[1]

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