Molecular cloning of the human Nurr1 gene: characterization of the human gene and cDNAs

被引:45
作者
Ichinose, H [1 ]
Ohye, T
Suzuki, T
Sumi-Ichinose, C
Nomura, T
Hagino, Y
Nagatsu, T
机构
[1] Fujita Hlth Univ, Inst Comprehens Med Sci, Aichi 4701192, Japan
[2] Fujita Hlth Univ, Sch Med, Dept Pharmacol, Aichi 4701192, Japan
关键词
cDNA; dopamine; exons; introns; orphan receptors; Parkinson's disease; schizophrenia;
D O I
10.1016/S0378-1119(99)00065-7
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Nurr1 is a member of the nuclear receptor superfamily of transcription factors that is expressed predominantly in the central nervous system, including developing dopaminergic neurons. Recently, it was demonstrated that Nurr1 is critical for midbrain dopaminergic cell differentiation. In order to investigate a possible relation of Nurr1 with the pathogenesis of Parkinson's disease or other neuropsychiatric disorders, we have cloned and characterized the human Nurr1 gene. The gene exists as a single copy in the human genome and comprises eight exons spanning 8 kb. We determined the complete nucleotide sequence and flanking regions of the gene. Potential regulatory regions included consensus binding sites for NF-kappa B, CREB, and Sp1. Isolation of human Nurr1 cDNAs from fetal brain suggested the presence of a new splicing variant of Nurr1 in the human brain. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:233 / 239
页数:7
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