About the possible origin and mechanism of circulating DNA - Apoptosis and active DNA release

被引：590

作者：

Stroun, M

Lyautey, J

Lederrey, C

Olson-Sand, A

Anker, P

机构：

[1] Fac Sci, Lab Biochim & Physiol Vegetale, CH-1211 Geneva, Switzerland

[2] Ares Serono, CH-1211 Geneva, Switzerland

来源：

CLINICA CHIMICA ACTA | 2001年 / 313卷 / 1-2期

关键词：

circulating DNA; apoptosis; active release;

D O I：

10.1016/S0009-8981(01)00665-9

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: In addition to cell lysis, apoptosis has been advanced as the origin of circulating DNA on the basis of several observations. Plasma or serum DNA often presents a ladder pattern reminiscent of that displayed by apoptotic cells when subjected to electrophoresis. However, the phenomenon of active release of DNA from cells might also be expected to result in a ladder pattern on electrophoresis. Non-dividing cells, such as lymphocytes, frog auricles and cultured cell lines including HL-60, spontaneously release a nucleoprotein complex within a homeostatic system in which newly synthesized DNA is preferentially released. Conclusion: In relation to DNA synthesis, the phenomenon of extracellular DNA in different culture conditions favors apoptosis or spontaneous active DNA release. (C) 2001 Published by Elsevier Science B.V.

引用

页码：139 / 142

页数：4

共 14 条

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