Genome-wide analysis of mutations in mutant lineages selected following fast-neutron irradiation mutagenesis of Arabidopsis thaliana

被引:115
作者
Belfield, Eric J. [1 ]
Gan, Xiangchao [1 ,2 ]
Mithani, Aziz [1 ]
Brown, Carly [1 ]
Jiang, Caifu [1 ]
Franklin, Keara [3 ]
Alvey, Elizabeth [4 ]
Wibowo, Anjar [1 ,5 ]
Jung, Marko [6 ]
Bailey, Kit [1 ]
Kalwani, Sharan [7 ]
Ragoussis, Jiannis [2 ]
Mott, Richard [2 ]
Harberd, Nicholas P. [1 ]
机构
[1] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
[2] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford OX3 78N, England
[3] Univ Bristol, Sch Biol Sci, Bristol BS8 1UG, Avon, England
[4] Univ Cambridge, Dept Plant Sci, Cambridge C82 3EA, England
[5] King Abdullah Univ Sci & Technol, Ctr Plant Stress Genom & Technol, Thuwal 23955690, Saudi Arabia
[6] Univ Oxford, Oxford E Res Ctr, Oxford OX1 3QG, England
[7] King Abdullah Univ Sci & Technol, Dept Informat Technol, Thuwal 23955690, Saudi Arabia
基金
英国生物技术与生命科学研究理事会; 英国惠康基金;
关键词
MOLECULAR-SPECTRUM; LIGHT CONTROL; CARCINOGENESIS; ENCODES; LOCUS;
D O I
10.1101/gr.131474.111
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ionizing radiation has long been known to induce heritable mutagenic change in DNA sequence. However, the genome-wide effect of radiation is not well understood. Here we report the molecular properties and frequency of mutations in phenotypically selected mutant lines isolated following exposure of the genetic model flowering plant Arabidopsis thaliana to fast neutrons (FNs). Previous studies suggested that FNs predominantly induce deletions longer than a kilobase in A. thaliana. However, we found a higher frequency of single base substitution than deletion mutations. While the overall frequency and molecular spectrum of fast-neutron (FN)-induced single base substitutions differed substantially from those of "background" mutations arising spontaneously in laboratory-grown plants, G:C>A:T transitions were favored in both. We found that FN-induced G:C>A:T transitions were concentrated at pyrimidine dinucleotide sites, suggesting that FNs promote the formation of mutational covalent linkages between adjacent pyrimidine residues. In addition, we found that FNs induced more single base than large deletions, and that these single base deletions were possibly caused by replication slippage. Our observations provide an initial picture of the genome-wide molecular profile of mutations induced in A. thaliana by FN irradiation and are particularly informative of the nature and extent of genome-wide mutation in lines selected on the basis of mutant phenotypes from FN-mutagenized A. thaliana populations.
引用
收藏
页码:1306 / 1315
页数:10
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