Analysis of dissociated single neurons by simple and semi-quantitative RT-PCR (reverse transcription and polymerase chain reaction)

被引：7

作者：

Andoh, T

Tohda, C

Kuraishi, Y

机构：

[1] TOYAMA MED & PHARMACEUT UNIV,FAC PHARMACEUT SCI,DEPT APPL PHARMACOL,TOYAMA 93001,JAPAN

[2] TOYAMA MED & PHARMACEUT UNIV,RES INST WAKAN YAKU,ANAL RES CTR ETHNOMED,TOYAMA 93001,JAPAN

来源：

JAPANESE JOURNAL OF PHARMACOLOGY | 1997年 / 74卷 / 01期

关键词：

histamine H-1-receptor mRNA; single whole-cell PCR; primary sensory neuron;

D O I：

10.1254/jjp.74.121

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H-1-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.

引用

页码：121 / 123

页数：3

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