Expression mapping of ectonucleotide pyrophosphatase/phosphodiesterase 1-3 (E-NPP1-3) in different brain structures during rat development

被引：15

作者：

Cognato, Giana de Paula ^{[2
]}

Czepielewski, Rafael Sanguinetti ^{[1
]}

Freitas Sarkis, Joao Jose ^{[2
]}

Bogo, Mauricio Reis ^{[3
]}

Bonan, Carla Denise ^{[1
]}

机构：

[1] Pontificia Univ Catolica Rio Grande do Sul, Fac Biociencias, Dept Biodiversidade, Lab Biol Genom & Mol, BR-9061900 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Dept Bioquim, Porto Alegre, RS, Brazil

[3] Pontificia Univ Catolica Rio Grande do Sul, Fac Biociencias, Dept Biol Celular & Mol, Lab Neuroquim & Psicofarmacol, BR-9061900 Porto Alegre, RS, Brazil

来源：

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE | 2008年 / 26卷 / 06期

关键词：

developing brain; E-NPP; LPA; autotoxin; purinergic signaling; ATP;

D O I：

10.1016/j.ijdevneu.2008.05.001

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ecto-nucleotide pyrophosphatases/phosphodiesterases (E-NPPs) are membrane-bound ecto-enzymes involved in the modulation of purinergic signaling. Important physiological roles related to brain development have been associated to purinergic neurotransmission. NPP1, two splice isoforms of NPP2, and NPP3 have already been identified in adult rat brain. However, there are no studies evaluating the mRNA expression of these NPP members during the brain development. The effort of the present study was to map NPP gene expression pattern in olfactory bulb, hippocampus, cerebral cortex, striatum, and cerebellum at crucial ages for rat development (7, 14, 21, 60, and 150 days old) by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) strategy. Our results demonstrated an increase in the relative expression of NPP1 throughout the aging in all structures analyzed, except in hippocampus, where the higher expression has been detected in 14 days old rats. Both NPP2 isoforms have shown a similar pattern of expression among all structures. The relative expression of NPP3 decreased during the aging mainly on cerebellum, hippocampus, and olfactory bulb. Altogether, the different patterns of NPP gene expression during rat brain development reinforce the idea that each enzyme may play a distinct role on modulating the purinergic signaling throughout aging. (C) 2008 ISDN. Published by Elsevier Ltd. All rights reserved.

引用

页码：593 / 598

页数：6

共 28 条

[1] Immunolocalization of ecto-nucleotide pyrophosphatase/phosphodiesterase 1 (NPP1) in the rat forebrain [J].